24822-33-1Relevant articles and documents
Production of keto-disaccharides from aldo-disaccharides in subcritical aqueous ethanol
Gao, Da-Ming,Kobayashi, Takashi,Adachi, Shuji
, p. 998 - 1005 (2016/05/09)
Isomerization of disaccharides (maltose, isomaltose, cellobiose, lactose, melibiose, palatinose, sucrose, and trehalose) was investigated in subcritical aqueous ethanol. A marked increase in the isomerization of aldo-disaccharides to keto-disaccharides was noted and their hydrolytic reactions were suppressed with increasing ethanol concentration. Under any study condition, the maximum yield of keto-disaccharides produced from aldo-disaccharides linked by β-glycosidic bond was higher than that produced from aldo-disaccharides linked by α-glycosidic bond. Palatinose, a keto-disaccharide, mainly underwent decomposition rather than isomerization in subcritical water and subcritical aqueous ethanol. No isomerization was noted for the non-reducing disaccharides trehalose and sucrose. The rate constant of maltose to maltulose isomerization almost doubled by changing solvent from sub-critical water to 80 wt% aqueous ethanol at 220°C. Increased maltose monohydrate concentration in feed decreased the conversion of maltose and the maximum yield of maltulose, but increased the productivity of maltulose. The maximum productivity of maltulose was ca. 41 g/(h kg-solution).
Production of galacto-oligosaccharides by the β-galactosidase from kluyveromyces lactis: Comparative analysis of permeabilized cells versus soluble enzyme
Rodriguez-Colinas, Barbara,De Abreu, Miguel A.,Fernandez-Arrojo, Lucia,De Beer, Roseri,Poveda, Ana,Jimenez-Barbero, Jesus,Haltrich, Dietmar,Ballesteros Olmo, Antonio O.,Fernandez-Lobato, Maria,Plou, Francisco J.
experimental part, p. 10477 - 10484 (2012/07/17)
The transgalactosylation activity of Kluyveromyces lactis cells was studied in detail. Cells were permeabilized with ethanol and further lyophilized to facilitate the transit of substrates and products. The resulting biocatalyst was assayed for the synthesis of galacto-oligosaccharides (GOS) and compared with two soluble β-galactosidases from K. lactis (Lactozym 3000 L HP G and Maxilact LGX 5000). Using 400 g/L lactose, the maximum GOS yield, measured by HPAEC-PAD analysis, was 177 g/L (44% w/w of total carbohydrates). The major products synthesized were the disaccharides 6-galactobiose [Gal-β(1?6)-Gal] and allolactose [Gal-β(1?6)-Glc], as well as the trisaccharide 6-galactosyl-lactose [Gal-β(1?6)-Gal-β(1?4)-Glc], which was characterized by MS and 2D NMR. Structural characterization of another synthesized disaccharide, Gal-β(1?3)-Glc, was carried out. GOS yield obtained with soluble β-galactosidases was slightly lower (160 g/L for Lactozym 3000 L HP G and 154 g/L for Maxilact LGX 5000); however, the typical profile ith a maximum GOS concentration followed by partial hydrolysis of the newly formed oligosaccharides was not observed with the soluble enzymes. Results were correlated with the higher stability of β-galactosidase when permeabilized whole cells were used.
Identification of oligosaccharides formed during stachyose hydrolysis by pectinex ultra SP-L
Montilla, Antonia,Corzo, Nieves,Olano, Agustin,Jimen, Maria Luisa
experimental part, p. 5007 - 5013 (2010/06/14)
The commercial enzyme preparation Pectinex Ultra SP-L containing fructosyltransferase activity was used to hydrolyze stachyose. During this reaction, besides the formation of mono-, di-, and trisaccharides (DP 3), the presence of one pentasacch
