24901-99-3

Basic information

• Product Name: 1-arseno-3-phosphoglycerate
• Synonyms: 1-arseno-3-phosphoglycerate
• CAS NO: 24901-99-3
• Molecular Weight: 309.986
• Mol File: 24901-99-3.mol
• Article Data: 1

Chemical Properties

• CAS DataBase Reference: 1-arseno-3-phosphoglycerate(CAS DataBase Reference)
• NIST Chemistry Reference: 1-arseno-3-phosphoglycerate(24901-99-3)
• EPA Substance Registry System: 1-arseno-3-phosphoglycerate(24901-99-3)

Safety Data

• Hazardous Substances Data: 24901-99-3(Hazardous Substances Data)

Upstream product

• 142-10-9 DL-glyceraldehyde 3-phosphate 

Relevant articles and documents

Kinetic and mechanistic characterization of the glyceraldehyde 3-phosphate dehydrogenase from Mycobacterium tuberculosis

Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is a glycolytic protein responsible for the conversion of glyceraldehyde 3-phosphate (G3P), inorganic phosphate and nicotinamide adenine dinucleotide (NAD+) to 1,3-bisphosphoglycerate (1,3-BPG) and the reduced form of nicotinamide adenine dinucleotide (NADH). Here we report the characterization of GAPDH from Mycobacterium tuberculosis (Mtb). This enzyme exhibits a kinetic mechanism in which first NAD+, then G3P bind to the active site resulting in the formation of a covalently bound thiohemiacetal intermediate. After oxidation of the thiohemiacetal and subsequent nucleotide exchange (NADH off, NAD+ on), the binding of inorganic phosphate and phosphorolysis yields the product 1,3-BPG. Mutagenesis and iodoacetamide (IAM) inactivation studies reveal the conserved C158 to be responsible for nucleophilic catalysis and that the conserved H185 to act as a catalytic base. Primary, solvent and multiple kinetic isotope effects revealed that the first half-reaction is rate limiting and utilizes a step-wise mechanism for thiohemiacetal oxidation via a transient alkoxide to promote hydride transfer and thioester formation.