27821-45-0 Usage
Description
Uridine-5'-diphosphoglucose disodium salt, also known as UDP-GlcNAc, is an organometallic compound that serves as an essential substrate in various biological processes. It is a nucleotide sugar that plays a crucial role in the biosynthesis of glycoproteins, glycolipids, and the modification of nucleic acids. UDP-GlcNAc is involved in cellular metabolism and signaling pathways, making it a significant molecule in the field of biochemistry and molecular biology.
Uses
Used in Biochemical Research:
Uridine-5'-diphosphoglucose disodium salt is used as a research tool for studying nucleic acid (RNA) biosynthesis and cell signaling. It is particularly useful in understanding the role of P2Y6 receptor agonists and GPR105 receptor inhibitors in cellular processes.
Used in Metabolite Quantification:
In the field of metabolomics, Uridine-5'-diphosphoglucose disodium salt is used as a standard for quantification of metabolite levels in murine tumor interstitial fluid by liquid chromatography-mass spectrometry (LC/MS). This application aids in the accurate measurement and analysis of metabolites, contributing to a better understanding of metabolic pathways in cancer research.
Used in Nucleotide Analysis:
Uridine-5'-diphosphoglucose disodium salt is utilized as a UDP standard for nucleotide analysis by liquid chromatography-high resolution mass spectrometry (LC-HRMS). This technique is vital for the identification and quantification of nucleotides in various biological samples, which can be applied to study the role of nucleotides in cellular processes and diseases.
Used in Cell Biology Studies:
Uridine-5'-diphosphoglucose disodium salt is employed in the treatment of E6.5 retina explants in vitro to study its effect on the entry and elongation of microglial cells into the embryonic quail retina. This application helps researchers understand the role of UDP-GlcNAc in cellular migration and development, particularly in the context of the nervous system.
Biochem/physiol Actions
Uridine 5′-diphosphate (UDP) is an endogenous signaling molecule produced by damaged cells to attract macrophages. In response to neuronal damage, UDP promotes chemotaxis and chemokinesis in microglial cells. UDP serves as a ligand for P2Y receptors. UDP and uridine 5′-triphosphate (UTP) may be used in studies on nucleic acid (RNA) biosynthesis and cell signaling. UDP is a nucleotide that upon phosphorylation to UTP becomes a substrate for enzymes such as RNA polymerase(s) and GTPases. These enzymes are involved in a wide range of applications from the synthesis of RNA to the regulation of G-coupled protein receptors (GPCR) and cell signaling molecules such as Rho-signaling via guanine nucleotide exchange factors (GEF).
Purification Methods
Crystallise it from MeOH. It may contain some Ba salt(s); hence stir it with Amberlite IR-120 cation exchanger (25mL, wet resin, 15-50 mesh in H+ form) in H2O (50mL) until the nucleotide dissolves. Filter, wash resin with H2O until the eluate is neutral. Combine the filtrate and washings, and adjust the pH to 8.0 with 2.0 M aqueous NaOH. Concentrate it in vacuo to a small volume (~ 20mL), and add Me2CO dropwise until crystallisation begins. Cool to 0o, and the shiny plates of di Na uridine-5’-phosphate dihydrate are filtered off and dried over P2O5 at 25o/0.1mm for 24hours (>76% recovery). UV: max at 262nm ( 10,000 M-1cm -1) in 0.1 M HCl. [Boon et al. J Chem Soc 408 1950, Smith Biochemical Preparations 8 130 1960.] [Beilstein 24 IV 1214.]
Check Digit Verification of cas no
The CAS Registry Mumber 27821-45-0 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 2,7,8,2 and 1 respectively; the second part has 2 digits, 4 and 5 respectively.
Calculate Digit Verification of CAS Registry Number 27821-45:
(7*2)+(6*7)+(5*8)+(4*2)+(3*1)+(2*4)+(1*5)=120
120 % 10 = 0
So 27821-45-0 is a valid CAS Registry Number.
