2887-07-2Relevant articles and documents
Pd(II)-Mediated C?H Activation for Cysteine Bioconjugation
Tilden, James A. R.,Lubben, Anneke T.,Reeksting, Shaun B.,Kociok-K?hn, Gabriele,Frost, Christopher G.
supporting information, (2022/01/19)
Selective bioconjugation remains a significant challenge for the synthetic chemist due to the stringent reaction conditions required by biomolecules coupled with their high degree of functionality. The current trailblazer of transition-metal mediated bioconjugation chemistry involves the use of Pd(II) complexes prepared via an oxidative addition process. Herein, the preparation of Pd(II) complexes for cysteine bioconjugation via a facile C?H activation process is reported. These complexes show bioconjugation efficiency competitive with what is seen in the current literature, with a user-friendly synthesis, common Pd(II) sources, and a more cost-effective ligand. Furthermore, these complexes need not be isolated, and still achieve high conversion efficiency and selectivity of a model peptide. These complexes also demonstrate the ability to selectively arylate a single surface cysteine residue on a model protein substrate, further demonstrating their utility.
Fluorescent probe for simultaneously detecting pH values of beta-galactosidase and lysosome as well as preparation method and application of fluorescent probe
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Paragraph 0017; 0018, (2021/08/11)
The invention discloses a compound shown in a structural general formula (I) and a preparation method thereof, the compound can simultaneously detect pH of beta-galactosidase and lysosome, the compound is a single-molecule ratio type two-dimensional fluor
Fluorescent probe for rapidly detecting β - galactosidase and preparation method and application thereof
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Paragraph 0047; 0051-0053, (2021/10/27)
The invention relates to a fluorescent probe for rapidly detecting β - galactosidase and a preparation method and application thereof. The fluorescent probe for rapidly detecting β - galactosidase has a chemical structural formula as shown in (I). The nea