29681-41-2Relevant articles and documents
Synthesis of substituted terpyridine ligands for use in protein purification
Lin, Chih-Pei,Florio, Pas,Campi, Eva M.,Zhang, Chunfang,Fredericks, Dale P.,Saito, Kei,Jackson, W. Roy,Hearn, Milton T.W.
, p. 8520 - 8531 (2014/12/11)
A number of 4,4″-disubstituted terpyridines bearing a 4′-thioethanamine linker, and regioisomers thereof, have been synthesised from 4-substituted picolinates. The substituted terpyridines were immobilised onto epoxy-activated Sepharose FF gel, creating functionalised solid supports for the fractionation of proteins - including antibodies - by mixed mode affinity chromatography. The metal chelating properties of the immobilised ligand render the stationary phase also amenable for use in immobilised metal-ion affinity chromatography (IMAC), and have been demonstrated with the purification of suitably tagged green fluorescent protein (GFP).