312-45-8 Usage
Description
HEMICHOLINIUM-3 is a compound that acts as an acetylcholine stores depletor. It is known for its ability to inhibit the high-affinity uptake of choline, which is a precursor for the synthesis of acetylcholine. This property makes it a valuable tool in the study of cholinergic systems and the regulation of acetylcholine synthesis.
Uses
Used in Neuroscience Research:
HEMICHOLINIUM-3 is used as a research tool for studying cholinergic systems. It serves as a cholinergic probe to deplete acetylcholine stores, which helps researchers understand the role of acetylcholine in various neurological processes and disorders.
Used in Cell Culture Studies:
HEMICHOLINIUM-3 is used in the preparation of Krebs-HC-3 buffer, which is utilized to study its effects on choline uptake and choline acetyltransferase activity in differentiated human neuroblastoma (SK-N-SH) cells. This application aids in the investigation of the mechanisms underlying cholinergic neurotransmission and the potential therapeutic targets for related conditions.
Biochem/physiol Actions
Hemicholinium-3 plays a role in blocking the neuronal choline uptake thereby inhibiting acetylcholine synthesis in the brain.
Check Digit Verification of cas no
The CAS Registry Mumber 312-45-8 includes 6 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 3 digits, 3,1 and 2 respectively; the second part has 2 digits, 4 and 5 respectively.
Calculate Digit Verification of CAS Registry Number 312-45:
(5*3)+(4*1)+(3*2)+(2*4)+(1*5)=38
38 % 10 = 8
So 312-45-8 is a valid CAS Registry Number.
312-45-8Relevant articles and documents
Synthesis and biodistribution of new radiolabeled high-affinity choline transporter inhibitors [11C]hemicholinium-3 and [18F]hemicholinium-3
Zheng, Qi-Huang,Gao, Mingzhang,Mock, Bruce H.,Wang, Shuyan,Hara, Toshihiko,Nazih, Rachid,Miller, Michael A.,Receveur, Tim J.,Lopshire, John C.,Groh, William J.,Zipes, Douglas P.,Hutchins, Gary D.,DeGrado, Timothy R.
, p. 2220 - 2224 (2007/10/03)
The high-affinity choline transporter (CHT1) system is an attractive target for the development of positron emission tomography (PET) biomarkers to probe brain, cardiac, and cancer diseases. An efficient and convenient synthesis of new radiolabeled CHT1 inhibitors [11C]hemicholinium-3 and [18F]hemicholinium-3 by solid-phase extraction (SPE) technique using a cation-exchange CM Sep-Pak cartridge has been well developed. The preliminary evaluation of both tracers through biodistribution studies in 9L-glioma rats has been performed, and the uptakes in the heart and tumor were observed, while very low brain uptake was seen.