3248-91-7 Usage
Chemical Properties
solid
Uses
Textiles and leather industries, red dye, pharmaceutical.
Definition
ChEBI: A hydrochloride that is the monohydrochloride of 4-[(4-aminophenyl)(4-iminocyclohexa-2,5-dien-1-ylidene)methyl]-2-methylaniline. One of the minor constituents of Basic fuchsin, together with pararosanilin, rosanilin and magenta II.
Preparation
Commonly known as the New Magenta. 4 – (4-Amino-3-methylbenzyl)-2-methylbenzenamine with O-Methylaniline its hydrochloride salt is heated in the presence of an oxidizing agent.
General Description
The dry dye New fuchsin (C.I. 42520) for microscopy Certistain?, is used for the preparation of a staining solution that can be used for staining of histological tissue sections or other samples of human origin according to Ziehl-Neelsen, Gram staining as well as in combination with Schiff′s reagent. As a Certistain? dye it is chemically quality-certified according to highest standards and strict specifications. New fuchsin belongs to the group of triaminotriphenylmethane dyes.The dye is used in Ziehl-Neelsen and Gram staining as well as for methods with Schiff′s reagent.The cell wall of acid-fast bacteria (AFB) has a high proportion of wax and lipids and hence absorbs dyes only very slowly. As a measure to enhance the absorption of the fuchsin dye and thus the formation of the mycolate-fuchsin complex in the cell wall, the carbolfuchsin solution applied to the specimen is normally heated to evaporation point. If the Kinyoun carbolfuchsin solution is used, the heating of the solution is dispensable. Consequently, the release of hazardous phenolic vapors will be avoided.Once the AFB have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol. Accordingly, AFB are termed as acid- and alcohol-fast for staining, and are stained red in the microscopic visualization. Correspondingly, all non-acid-fast microorganisms are counterstained with an appropriate dye.Pretreatment of the specimens with Sputofluol? dissolves the bacteria from the surrounding viscid sputum and cell material. Sputofluol? also has a disinfectant effect, with the result that any microorganisms that are present are killed off. It is registered as IVD and certified as CE product. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
Hazard
Possible carcinogen.
Flammability and Explosibility
Notclassified
Properties and Applications
dark red light purple. Soluble in water and ethanol is red. The strong sulfuric acid for yellow until golden brown, diluted into light yellow. Dyes to join sodium hydroxide solution appears orange brown precipitation.
Standard
Light Fastness
Persperation Fastness
Ironing Fastness
Soaping
Fading
Stain
Fading
Stain
Fading
Stain
ISO
3
4
4-5
4-5
5
AATCC
3
Standard
Light Fastness
Fading
Stain
ISO
3
AATCC
3
Check Digit Verification of cas no
The CAS Registry Mumber 3248-91-7 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 3,2,4 and 8 respectively; the second part has 2 digits, 9 and 1 respectively.
Calculate Digit Verification of CAS Registry Number 3248-91:
(6*3)+(5*2)+(4*4)+(3*8)+(2*9)+(1*1)=87
87 % 10 = 7
So 3248-91-7 is a valid CAS Registry Number.
InChI:InChI=1/C20H19N3.C6H8O2.ClH.K/c1-13-12-16(6-11-19(13)23)20(14-2-7-17(21)8-3-14)15-4-9-18(22)10-5-15;1-2-3-4-5-6(7)8;;/h2-12,21H,22-23H2,1H3;2-5H,1H3,(H,7,8);1H;/q;;;+1/p-1/b20-14-,21-17?;3-2+,5-4+;;
