328273-17-2

Basic information

• Product Name: (3aS,4S,6aR)-N-[5-[(1,1-dimethylethoxy)carbonyl]aminopentyl]hexahydro-2-oxo-1H-thieno[3,4-d]imidazole-4-pentanamide
• Synonyms: (3aS,4S,6aR)-N-[5-[(1,1-dimethylethoxy)carbonyl]aminopentyl]hexahydro-2-oxo-1H-thieno[3,4-d]imidazole-4-pentanamide
• CAS NO: 328273-17-2
• Molecular Weight: 428.596
• Mol File: 328273-17-2.mol
• Article Data: 7

Chemical Properties

• CAS DataBase Reference: (3aS,4S,6aR)-N-[5-[(1,1-dimethylethoxy)carbonyl]aminopentyl]hexahydro-2-oxo-1H-thieno[3,4-d]imidazole-4-pentanamide(CAS DataBase Reference)
• NIST Chemistry Reference: (3aS,4S,6aR)-N-[5-[(1,1-dimethylethoxy)carbonyl]aminopentyl]hexahydro-2-oxo-1H-thieno[3,4-d]imidazole-4-pentanamide(328273-17-2)
• EPA Substance Registry System: (3aS,4S,6aR)-N-[5-[(1,1-dimethylethoxy)carbonyl]aminopentyl]hexahydro-2-oxo-1H-thieno[3,4-d]imidazole-4-pentanamide(328273-17-2)

Safety Data

• Hazardous Substances Data: 328273-17-2(Hazardous Substances Data)

Usage

Description

(3aS,4S,6aR)-N-[5-[(1,1-dimethylethoxy)carbonyl]aminopentyl]hexahydro-2-oxo-1H-thieno[3,4-d]imidazole-4-pentanamide is a synthetic thienoimidazole derivative with a complex molecular structure. It contains multiple functional groups, including a carbonyl, an amine, and a thienoimidazole ring. Its structure suggests potential interactions with biological targets, such as enzymes or receptors, indicating possible pharmaceutical applications.

Uses

Used in Pharmaceutical Industry:
(3aS,4S,6aR)-N-[5-[(1,1-dimethylethoxy)carbonyl]aminopentyl]hexahydro-2-oxo-1H-thieno[3,4-d]imidazole-4-pentanamide is used as a potential pharmaceutical candidate for various applications due to its complex molecular structure and the presence of multiple functional groups. Its potential interactions with biological targets, such as enzymes or receptors, make it a promising compound for further research and development in the pharmaceutical industry. However, additional studies are required to determine its specific biological activities and potential therapeutic uses.

Upstream product

• 51644-96-3 5-tert-butoxycarbonylamino-1-aminopentane 
• 58-85-5 biotin 

Downstream Products

• 288259-39-2 5-((+)-biotinamido)pentylamine trifluoroacetic acid 
• 1031858-01-1 C₄₈H₄₉BF₂N₄O₅S 
• 1031857-99-4 C₂₂H₃₀F₂N₄O₃S 
• 1031858-00-0 C₂₈H₄₁BF₂N₄O₅S 
• 115416-38-1 N-(5-aminopentyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazole-4-yl)pentanamide 

Relevant articles and documents

Fluorometric assay for tissue transglutaminase-mediated transamidation activity

Herein, we report the development of a direct discontinuous fluorometric transamidation assay for determining tissue transglutaminase (TG2) activity. In the assay reaction, TG2 catalyzes the formation of a biotin-fluorophore conjugate, using a fluorescent, high affinity γ-glutamyl donor substrate and a biotinylated amine as a γ-glutamyl acceptor substrate. After the reaction, the conjugate is fixed on streptavidin-coated beads and excess substrate is washed away, allowing the transamidation activity to be quantified by fluorescence measurement. This method was used to detect the activity of as little as 0.6 mU of purified TG2, and can be used for detection of activity from crude cellular lysates. Furthermore, this assay can be used for screening potential inhibitors and synthetic substrates, the latter of which was demonstrated herein.

PHOTOPROXIMITY PROFILING OF PROTEIN-PROTEIN INTERACTIONS IN CELLS

Photoactive probes and probe systems for detecting biological interactions are described. The photoactive probes include probes that combine both photocleavable and photoreactive moieties. The photoactive probe systems can include a first probe comprising a photocatalytic group and a second probe comprising a group that can act as a substrate for the reaction catalyzed by the photocatalytic group. The probes and probe systems can also include groups that can specifically bind to a binding partner on a biological entity of interest and a detectable group or a precursor thereof. The probes and probe systems can detect spatiotemporal interactions of proteins or cells. In some embodiments, the interactions can be detected in live cells. Also described are methods of detecting the biological interactions.

An improved synthesis of a fluorophosphonate-polyethylene glycol-biotin probe and its use against competitive substrates

The fluorophosphonate (FP) moiety attached to a biotin tag is a prototype chemical probe used to quantitatively analyze and enrich active serine hydrolases in complex proteomes in an approach called activity-based protein profiling (ABPP). In this study we have designed a novel synthetic route to a known FP probe linked by polyethylene glycol to a biotin tag (FP-PEG-biotin). Our route markedly increases the efficiency of the probe synthesis and overcomes several problems of a prior synthesis. As a proof of principle, FP-PEG-biotin was evaluated against isolated protein mixtures and different rat-tissue homogenates, showing its ability to specifically target serine hydrolases. We also assessed the ability of FP-PEG-biotin to compete with substrates that have high enzyme turnover rates. The reduced protein-band intensities resulting in these competition studies demonstrate a new application of FP-based probes seldom explored before.