3398-50-3 Usage
Description
5-bromouridine triphosphate is a pyrimidine ribonucleoside 5'-triphosphate with 5-bromouracil as the pyrimidine component. It is a modified nucleotide that plays a significant role in various molecular biology applications.
Usage:
Used in Molecular Biology:
5-bromouridine triphosphate is used as an incorporation agent for the detection and analysis of nucleic acids. It is particularly useful in techniques such as in situ hybridization, where it helps in identifying and localizing specific nucleic acid sequences within cells or tissues.
Used in DNA Sequencing:
In DNA sequencing, 5-bromouridine triphosphate is used as a chain-terminating agent. It is incorporated into the growing DNA strand during the sequencing process, leading to the termination of the strand at specific points. This allows for the determination of the sequence of nucleotides in the DNA molecule.
Used in Mutagenesis Studies:
5-bromouridine triphosphate is used as a mutagenic agent in genetic research. It can be incorporated into DNA during replication, leading to the introduction of mutations. This is useful for studying the effects of specific mutations on gene function and protein structure.
Used in Diagnostics:
In the field of diagnostics, 5-bromouridine triphosphate is used as a labeling agent for the detection of specific nucleic acid sequences. It can be used to label probes or primers, which can then be used to detect the presence of target sequences in samples, such as in PCR or other amplification techniques.
Used in Drug Development:
5-bromouridine triphosphate is used as a tool in the development of new drugs targeting nucleic acid metabolism. By studying the interactions of this modified nucleotide with various enzymes and cellular processes, researchers can gain insights into the mechanisms of action of potential therapeutic agents.
Check Digit Verification of cas no
The CAS Registry Mumber 3398-50-3 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 3,3,9 and 8 respectively; the second part has 2 digits, 5 and 0 respectively.
Calculate Digit Verification of CAS Registry Number 3398-50:
(6*3)+(5*3)+(4*9)+(3*8)+(2*5)+(1*0)=103
103 % 10 = 3
So 3398-50-3 is a valid CAS Registry Number.
3398-50-3Relevant articles and documents
Synthesis and structure-activity relationships of uracil nucleotide derivatives and analogues as agonists at human P2Y2, P2Y4, and P2Y6 receptors
El-Tayeb, Ali,Qi, Aidong,Müller, Christa E.
, p. 7076 - 7087 (2007/10/03)
A series of UTP, UDP, and UMP derivatives and analogues were synthesized and evaluated at the human pyrimidinergic P2Y receptor subtypes P2Y2, P2Y4, and P2Y6 stably expressed in 1321N1 astrocytoma cells. Substituents at N3
