37340-58-2

Basic information

• Product Name: CREATINE AMIDINOHYDROLASE
• Synonyms: EC 3.5.3.3;CREATINE AMIDINOHYDROLASE;CREATINASE;creatinase from actinobacillus species;creatinase from flavobacterium sp.;creatinase from pseudomonas species:*recombinant;CREATINASE FROM FLAVOBACTERIUM SPECIES;CREATINASE FROM FLAVOBACTERIUM SP., LYOP HIL., ~10 U/MG
• CAS NO: 37340-58-2
• Molecular Weight: 0
• EINECS: 253-471-1
• Mol File: 37340-58-2.mol
• Article Data: 0

Chemical Properties

• Appearance: white/lyophilized powder
• Storage Temp.: 2-8°C
• CAS DataBase Reference: CREATINE AMIDINOHYDROLASE(CAS DataBase Reference)
• NIST Chemistry Reference: CREATINE AMIDINOHYDROLASE(37340-58-2)
• EPA Substance Registry System: CREATINE AMIDINOHYDROLASE(37340-58-2)

Safety Data

• Safety Statements: 22-24/25
• WGK Germany: 3
• F: 10-21
• Hazardous Substances Data: 37340-58-2(Hazardous Substances Data)

Usage

Description

Creatine amidinohydrolase, also known as creatinase, is a homodimer enzyme that catalyzes the hydrolysis of creatine. It is composed of two monomer subunits and features two defined domains: the N-terminal and C-terminal domains. The C-terminal domain contains both α-helices and anti-parallel β-sheets within two structurally similar domains. A sulfhydryl group serves as the active site, and the enzyme's activity is metal-independent. Creatine amidinohydrolase plays a crucial role in the determination of creatinine levels in blood and urine, making it a clinically important enzyme.

Uses

Used in Clinical Diagnostics:
Creatine amidinohydrolase is used as a diagnostic tool for measuring creatinine levels in blood and urine samples. This application is essential for assessing kidney function and diagnosing various renal disorders, as creatinine is a waste product generated from muscle metabolism and is typically cleared by the kidneys.
Used in Research and Development:
In the field of research, creatine amidinohydrolase is employed as a valuable tool for studying the metabolism of creatine and its role in various physiological processes. This enzyme can also be utilized in the development of new therapeutic strategies targeting creatine metabolism-related conditions.
Used in Pharmaceutical Industry:
Creatine amidinohydrolase may be utilized in the pharmaceutical industry for the development of drugs that modulate creatine metabolism, which could potentially benefit patients with conditions related to muscle wasting or impaired renal function.

Biochem/physiol Actions

Creatinase is a homodimer with subunit molecular weights of approximately 45 kDa. It catalyzes the hydrolysis of creatine, in the presence of H2O, to form urea and sarcosine.