38404-93-2

Basic information

• Product Name: 5-Bromo-4-chloro-3-indolylphosphate
• Synonyms: 5-bromo-4-chloro-3-indoxyl phosphate;4-Chloro-5-bromo-1H-indole-3-ol phosphoric acid;5-Bromo-4-chloro-1H-indol-3-ol dihydrogen phosphate;5-Bromo-4-chloro-1H-indol-3-yldihydrogen phosphate;Phosphoric acid (4-chloro-5-bromo-1H-indole-3-yl) ester;Phosphoric acid 4-chloro-5-bromo-1H-indole-3-yl ester
• CAS NO: 38404-93-2
• Molecular Formula: C₈H₆BrClNO₄P
• Molecular Weight: 326.47
• Mol File: 38404-93-2.mol
• Article Data: 0

Chemical Properties

• Boiling Point: 580.2 °C at 760 mmHg
• Flash Point: 304.7 °C
• Appearance: /
• Density: 2.083 g/cm³
• CAS DataBase Reference: 5-Bromo-4-chloro-3-indolylphosphate(CAS DataBase Reference)
• NIST Chemistry Reference: 5-Bromo-4-chloro-3-indolylphosphate(38404-93-2)
• EPA Substance Registry System: 5-Bromo-4-chloro-3-indolylphosphate(38404-93-2)

Safety Data

• Hazardous Substances Data: 38404-93-2(Hazardous Substances Data)

Usage

Description

5-Bromo-4-chloro-3-indolylphosphate, also known as BCIP, is an aryl phosphate that is indoxyl phosphate in which the indole moiety is substituted at positions 4 and 5 by chlorine and bromine, respectively. It is a widely used colorimetric substrate for the detection of alkaline phosphatase enzyme activity.

Uses

Used in Molecular Biology and Biotechnology:
5-Bromo-4-chloro-3-indolylphosphate is used as a colorimetric substrate for the detection of alkaline phosphatase enzyme activity in various applications, such as Western blotting, immunohistochemistry, and colorimetric assays. It is particularly useful for the detection of alkaline phosphatase-tagged proteins or nucleic acids, providing a sensitive and reliable method for visualizing enzyme activity.
Used in Diagnostic Applications:
In the field of diagnostics, 5-Bromo-4-chloro-3-indolylphosphate is used as a reagent in enzyme-linked immunosorbent assays (ELISA) and other immunoassays to detect the presence of specific antibodies or antigens. The enzyme-linked signal amplification provided by BCIP allows for increased sensitivity and accuracy in diagnostic tests.
Used in Research and Development:
5-Bromo-4-chloro-3-indolylphosphate is used as a research tool in the development of new methods for detecting and quantifying alkaline phosphatase activity. Its unique properties make it an essential component in the design and optimization of novel assays and detection systems.
Used in Pharmaceutical Industry:
In the pharmaceutical industry, 5-Bromo-4-chloro-3-indolylphosphate is used in the development and testing of new drugs targeting alkaline phosphatase enzymes. Its ability to provide a colorimetric readout of enzyme activity makes it a valuable tool for high-throughput screening and drug discovery efforts.
Used in Environmental Monitoring:
5-Bromo-4-chloro-3-indolylphosphate is also used in environmental monitoring applications to detect the presence of alkaline phosphatase enzymes in various samples, such as soil, water, and air. This information can be used to assess the health of ecosystems and monitor the effectiveness of environmental remediation efforts.

Downstream Products

• 29245-44-1 5,5'-dibromo-4,4'-dichloro-1H,1'H-[2,2']biindolylidene-3,3'-dione