38597-12-5 Usage
Description
4-METHYLUMBELLIFERYL-ALPHA-D-GALACTOPYRANOSIDE, also known as 4-MU-α-D-Galactopyranoside, is a synthetic compound that serves as a fluorogenic substrate for the detection and measurement of α-galactosidase enzyme activity. It is an alpha-D-galactoside with a 4-methylumbelliferyl substituent at the anomeric position, which gives it its characteristic off-white solid appearance. When the α-galactosidase enzyme acts upon this substrate, it results in the release of a fluorescent product, 4-methylumbelliferone, which can be easily detected and quantified.
Uses
Used in Enzyme Assays:
4-METHYLUMBELLIFERYL-ALPHA-D-GALACTOPYRANOSIDE is used as a fluorogenic substrate for the assay of α-galactosidase, an enzyme that plays a crucial role in the metabolism of various glycosides. The detection of the fluorescent product, 4-methylumbelliferone, allows for the quantification of enzyme activity, which is essential in research and diagnostic applications.
Used in Cell Biology:
In Cell Biology, 4-METHYLUMBELLIFERYL-ALPHA-D-GALACTOPYRANOSIDE is used as a fluorescent substrate for α-galactosidase activity in cell extracts, lysosomes, and human blood serum. The lower pKa of the hydrolysis product enables its detection at physiological pH, making it a valuable tool for studying enzyme activity within cellular environments.
Used in Diagnostics:
In the Diagnostics industry, 4-METHYLUMBELLIFERYL-ALPHA-D-GALACTOPYRANOSIDE is used as a diagnostic tool for the detection and measurement of α-galactosidase enzyme activity. This is particularly important in the identification and monitoring of various lysosomal storage disorders, such as Fabry disease, where the enzyme's activity is compromised.
Used in Pharmaceutical Research:
In Pharmaceutical Research, 4-METHYLUMBELLIFERYL-ALPHA-D-GALACTOPYRANOSIDE is used as a research tool for the development of new drugs targeting α-galactosidase and related enzymes. By understanding the enzyme's activity and its role in various diseases, researchers can design more effective therapies for the treatment of these conditions.
Check Digit Verification of cas no
The CAS Registry Mumber 38597-12-5 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 3,8,5,9 and 7 respectively; the second part has 2 digits, 1 and 2 respectively.
Calculate Digit Verification of CAS Registry Number 38597-12:
(7*3)+(6*8)+(5*5)+(4*9)+(3*7)+(2*1)+(1*2)=155
155 % 10 = 5
So 38597-12-5 is a valid CAS Registry Number.
38597-12-5Relevant articles and documents
Based on 4 - methyl [...] synthesis method of a plurality of glycoside
-
Paragraph 0082; 0083, (2018/10/11)
The invention discloses a method for synthesizing various glucosides on a basis of 4-methylumbelliferone. According to the invention, a glycosyl donor peracetyl saccharide and a glycosyl acceptor 4-methylumbelliferone are subjected to a glycosylation reaction under room temperature or under heating with dichloromethane or 1,2-dichloroethane as a solvent and with the combined effect of Lewis acid boron trifluoride ethyl ether and organic alkali triethylamine or pyridine; and protecting groups are removed, such that various glucosides based on 4-methylumbelliferone can be obtained. The glucosides include 4-methylumbelliferone-beta-D-glucopyranosiduronide, 4-methylumbelliferone-beta-D-glucopyranoside, 4-methylumbelliferone-beta-D-xylopyranoside, 4-methylumbelliferone-beta-D-ribofuranoside, 4-methylumbelliferone-alpha-D-galactopyranoside, and 4-methylumbelliferone-alpha-D-mannopyranoside. The method is simple, and can produce a beta or alpha single-configuration target. A glycosylation reaction yield can reach 17-93%.
