3952-78-1 Usage
Description
Alizarin Fluorine Blue, also known as Alizarin-3-methyliminodiacetic acid, is a dihydroxyanthraquinone compound with hydroxy groups at C-1 and C-2 positions and a bis[(carboxymethyl)amino]methyl substituent at the 3-position. It is characterized by its brown-orange crystal appearance and is insoluble in water. Alizarin Fluorine Blue is a versatile compound with various applications in different fields, particularly in biological research and analysis.
Uses
Used in Analytical Chemistry:
Alizarin Fluorine Blue is used as a colorimetric dye for the detection of fluoride ions. It reacts with fluoride to form a lilac-blue complex, which can be quantified colorimetrically at 620 nm to determine fluoride concentration. This property makes it a valuable tool in the analysis of fluorine and other anions.
Used in Biological Research:
Alizarin Fluorine Blue is used as a staining agent in biological research to visualize the presence of mineralized bone matrix in preserved specimens from vertebrates. It is particularly useful for live staining, double-labeling in dynamic bone histomorphometry, and indicating new bone formation in various studies.
Used in Pharmaceutical Applications:
Alizarin Fluorine Blue has been identified as a potent inhibitor of protein-nucleic acid interactions, which can be beneficial in the development of new drugs and therapies targeting these interactions.
Used in Pigment Manufacturing:
Alizarin is the main ingredient for the manufacture of madder lake pigments, known to painters as Rose madder and Alizarin crimson. These pigments are widely used in the art and painting industry for their vibrant colors.
Used in Histomorphometry:
Alizarin Red, a variant of Alizarin Fluorine Blue, is used in a biochemical assay to determine the presence of calcific deposition by cells of an osteogenic lineage through colorimetric analysis. This application aids in understanding bone formation and mineralization processes in various organisms, including rats and medaka larvae.
Hazard
Anthraquinones are toxic by ingestion: vomiting, diarrhea, kidney and
liver damage, and coma; Deaths have been reported after children
ingested Rhamnus berries; Has antiretroviral activity;An irritant; Harmful by ingestion, inhalation, or skin
absorption; Toxic if swallowed.
Purification Methods
It is purified by suspending it in 0.1M NaOH (1g in 50mL), filtering the solution and extracting alizarin with 5 successive portions of CH2Cl2. Then add HCl dropwise to precipitate the reagent, stirring the solution in an ice bath. Filter the precipitate onto a glass filter, wash it with cold water and dry it in a vacuum desiccator over KOH [Ingman Talanta 20 135 1973, Beilstein 14 IV 931].
Check Digit Verification of cas no
The CAS Registry Mumber 3952-78-1 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 3,9,5 and 2 respectively; the second part has 2 digits, 7 and 8 respectively.
Calculate Digit Verification of CAS Registry Number 3952-78:
(6*3)+(5*9)+(4*5)+(3*2)+(2*7)+(1*8)=111
111 % 10 = 1
So 3952-78-1 is a valid CAS Registry Number.
InChI:InChI=1/C19H15NO8.2H2O/c21-13(22)7-20(8-14(23)24)6-9-5-12-15(19(28)16(9)25)18(27)11-4-2-1-3-10(11)17(12)26;;/h1-5,25,28H,6-8H2,(H,21,22)(H,23,24);2*1H2
