45103-58-0Relevant articles and documents
Improvement of solid material for affinity resins by application of long PEG spacers to capture the whole target complex of FK506
Mabuchi, Miyuki,Shimizu, Tadashi,Ueda, Masahiro,Mitamura, Kuniko,Ikegawa, Shigeo,Tanaka, Akito
supporting information, p. 2788 - 2792 (2015/06/08)
Solid materials for affinity resins bearing long PEG spacers between a functional group used for immobilization of a bio-active compound and the solid surface were synthesized to capture not only small target proteins but also large and/or complex target proteins. Solid materials with PEG1000 or PEG2000 as spacers, which bear a benzenesulfonamide derivative, exhibited excellent selectivity between the specific binding protein carbonic anhydrase type II (CAII) and non-specific ones. These materials also exhibited efficacy in capturing a particular target at a maximal amount. Affinity resins using solid materials with PEG1000 or PEG2000 spacers, bear a FK506 derivative, successfully captured the whole target complex of specific binding proteins at the silver staining level, while all previously known affinity resins with solid materials failed to achieve this objective. These novel affinity resins captured other specific binding proteins such as dynamin and neurocalcin δ as well.
Unique associative properties of copolymers of sodium acrylate and oligo(ethylene oxide) alkyl ether methacrylates in water
Tomatsu, Itsuro,Hashidzume, Akihito,Yusa, Shin-Ichi,Morishima, Yotaro
, p. 7837 - 7844 (2008/02/01)
A series of random copolymers of sodium acrylate and oligo(ethylene oxide) alkyl ether methacrylates (CnEmMA) with different lengths of ethylene oxide (EO) and alkyl groups were prepared by free-radical copolymerization at varying copolymer compositions. The lengths of the EO units (the number of EO units) (m) and the numbers of carbon atoms in the alkyl groups (n) ranged fro'm 0 to 8.7 and 1 to 6, respectively. The copolymers with n = 1 and m = 1-8.7 exhibited a marked increase in solution viscosity at polymer concentrations (Cp) higher than their overlap concentrations (C*) when the CnEmMA contents (x) in the copolymers were in a certain limited range. Namely, there was an optimum x value that yielded the highest viscosity as a consequence of the competition between inter- and intrapolymer associations; the maximum viscosities occurred around x ≈ 25, 15, 10, 7, and 3 mol % for m = 1, 2, 3, 4.2, and 8.7, respectively. The maximum viscosity decreased significantly as n was increased on going from 1 to 6, and for the copolymers with n = 6, no increase in the viscosity occurred, a trend opposite to what is expected to interpolymer hydrophobic associations. When Cp > C*, steady-shear viscosity depended on the nature of countercations; the viscosities were found to be higher in the order Li + > Na+ ? NH4+, whereas reduced viscosity in dilute regime (Cp C*) was independent of the species of the cations. Rheological properties were found to be typical of transient networks formed through very weak interpolymer associations. Thus, the large increase in solution viscosity was explained by simultaneous interactions of countercations with EO units via coordination and with the polyanion via counterion condensation.
