452979-80-5Relevant articles and documents
The chameleon of retaining glycoside hydrolases and retaining glycosyl transferases: The catalytic nucleophile
Stick, Robert V.,Watts, Andrew G.
, p. 541 - 554 (2002)
This paper addresses the existence and role of a catalytic nucleophile in retaining glycoside hydrolases and retaining glycosyl transferases. Although the former has now been established beyond doubt, such is not the case with the latter. We report reliable procedures for the synthesis of various 2-deoxy-2-fluoro glycosyl nucleoside diphosphates, useful donor analogues for the study of the mechanism of action of retaining glycosyl transferases.