4631-12-3Relevant articles and documents
In vivo neurochemical monitoring using benzoyl chloride derivatization and liquid chromatography-mass spectrometry
Song, Peng,Mabrouk, Omar S.,Hershey, Neil D.,Kennedy, Robert T.
experimental part, p. 412 - 419 (2012/03/11)
In vivo neurochemical monitoring using microdialysis sampling is important in neuroscience because it allows correlation of neurotransmission with behavior, disease state, and drug concentrations in the intact brain. A significant limitation of current practice is that different assays are utilized for measuring each class of neurotransmitter. We present a high performance liquid chromatography (HPLC)-tandem mass spectrometry method that utilizes benzoyl chloride for determination of the most common low molecular weight neurotransmitters and metabolites. In this method, 17 analytes were separated in 8 min. The limit of detection was 0.03-0.2 nM for monoamine neurotransmitters, 0.05-11 nM for monoamine metabolites, 2-250 nM for amino acids, 0.5 nM for acetylcholine, 2 nM for histamine, and 25 nM for adenosine at sample volume of 5 μL. Relative standard deviation for repeated analysis at concentrations expected in vivo averaged 7% (n = 3). Commercially available 13C benzoyl chloride was used to generate isotope-labeled internal standards for improved quantification. To demonstrate utility of the method for study of small brain regions, the GABAA receptor antagonist bicuculline (50 μM) was infused into a rat ventral tegmental area while recording neurotransmitter concentration locally and in nucleus accumbens, revealing complex GABAergic control over mesolimbic processes. To demonstrate high temporal resolution monitoring, samples were collected every 60 s while neostigmine, an acetylcholine esterase inhibitor, was infused into the medial prefrontal cortex. This experiment revealed selective positive control of acetylcholine over cortical glutamate.
Reactivity of the Amino Groups of α-Amino Acids and Dipeptides in Reaction with Benzoyl Chloride in a Dioxane-Water Solvent
Kuritsyn, L. V.,Kalinina, N. V.
, p. 767 - 770 (2007/10/02)
The kinetic characteristics (rate constants, activation energies, and entropies of activation) for the reaction of a series of α-amino acids and dipeptides with benzoyl chloride in aqueous dioxane are presented.The amino group in the investigated α-amino acids has high reactivity 4 liter/mole*sec>; the dipeptides have lower reactivity 3 liter/mole*sec>.
Studies on Potential Antibacterial and Chelating Agents: Part I - Synthesis, Physicochemical Properties and Antibacterial Screening of Some Benzimidazoles
Nandi, M. M.,Ray, Ruma
, p. 222 - 224 (2007/10/02)
Six properly substituted benzimidazoles (I-VI) suitable for chelation have been synthesised from benzoylamino acids by condensation with o-phenylenediamine following Phillips and fusion methods.Dissociation constants of the benzoyl derivatives of glycine, (dl)-α-alanine, β-alanine, L-(-)-aspartic acid, L-(+)-glutamic acid and the six protonated benzamidobenzimidazoles (I-VI) in water-dioxane (1:1) at constant ionic strength (μ=0.5) at 20 deg C +/- 0.1 deg C have been determined by potentiometric titration.Structures of I-VI have been established on the basis of their elemental analysis, synthetic route, IR and PMR spectra and pK* values.Some of the compounds show significant antibacterial activity against gram + ve organisms only whereas corresponding sulphonamidobenzimidazoles are active against the gram - ve organism E. coli.
