4829-64-5Relevant articles and documents
Synthesis and physicochemical studies of partially phosphate-methylated oligodeoxyribonucleotides
Vinogradov,Asseline,Thuong
, p. 5899 - 5902 (1993)
Partially phosphate-methylated oligodeoxyribonucleotides have been synthesized on an oxalyl-CPG derivatized support using an isopropoxyacetyl group for the protection of the exocyclic amine of the nucleic bases. Hybridization properties with the target sequence have been studied by absorption spectroscopy.
O-selective condensation using P-N bond cleavage in RNA synthesis without base protection
Ohkubo, Akihiro,Kuwayama, Yasukazu,Kudo, Tomomi,Tsunoda, Hirosuke,Seio, Kohji,Sekine, Mitsuo
supporting information; experimental part, p. 2793 - 2796 (2009/06/06)
(Chemical Equation Presented) In RNA synthesis without base protection, a new method for O-selective condensation with more than 99% selectivity was developed by 6-nitro-HOBt-mediated cleavage of undesired P(III)-N bonds on nucleobase moieties. Moreover, we for the first time succeeded in synthesizing oligoRNAs without base protection.
A new strategy for the synthesis of oligodeoxynucleotides directed towards perfect O-selective internucleotidic bond formation without base protection
Ohkubo, Akihiro,Seio, Kohji,Sekine, Mitsuo
, p. 363 - 366 (2007/10/03)
Deoxyadenosine and deoxycytidine have nucleophilic amino groups so that the undesired N-phosphitylation of these amino groups occurred in the previous phosphoramidite methods without base protection. We report that the N-phosphitylation could be considerably suppressed in our new HOBt-mediated coupling strategy via phosphite intermediates as reactive species. Thus, 99.7-99.9% O-selective internucleotidic bond formation was achieved.
Synthesis and nuclease stability of dinucleotides containing an anti conformationally constrained acyclic thymidine
Hsu, Ling-Yih,Yang, Kuo-Tsao
, p. 2031 - 2042 (2007/10/03)
Novel heterodimers containing an anti conformationally constrained acyclic thymidine were prepared and the nuclease resistance of the modified dinucleotides were studied.