5043-03-8Relevant articles and documents
Rational Design of a Two-Photon Fluorogenic Probe for Visualizing Monoamine Oxidase A Activity in Human Glioma Tissues
Fang, Haixiao,Huang, Wei,Li, Lin,Li, Zheng,Ma, Bo,Ni, Yun,Shi, Riri,Wu, Qiong,Yang, Naidi,Yang, Xuekang,Yao, Shao Q.,Yu, Changmin,Zhang, Chengwu,Zhang, Hang
, p. 7536 - 7541 (2020)
Monoamine oxidases have two functionally distinct but structurally similar isoforms (MAO-A and MAO-B). The ability to differentiate them by using fluorescence detection/imaging technology is of significant biological relevance, but highly challenging with available chemical tools. Herein, we report the first MAO-A-specific two-photon fluorogenic probe (F1), capable of selective imaging of endogenous MAO-A enzymatic activities from a variety of biological samples, including MAO-A-expressing neuronal SY-SY5Y cells, the brain of tumor-bearing mice and human Glioma tissues by using two-photon fluorescence microscopy (TPFM) with minimal cytotoxicity.
ICT-based fluorescent ratiometric probe for monitoring mitochondrial peroxynitrite in living cells
Du, Yuting,Wang, Hongliang,Zhang, Ting,Wei, Wen,Guo, Minmin
supporting information, p. 12915 - 12921 (2021/08/03)
Peroxynitrite (ONOO?) is a reactive oxygen species (ROS) that causes serious damage to living cells and is the cause of a series of human diseases. It is reported that mitochondria are the major site of ONOO?production. Therefore, accurate and rapid detection of intracellular ONOO?is important in pathological processes. Herein, a mitochondria-targeted ratiometric fluorescent probe (DMANI) has been rationally designed based on the coumarin-hemicyanine hybrid framework by regulating the intramolecular charge transfer (ICT) effect. TheDMANIcan capture ONOO?at low concentrationsviadirect addition with the CC bond to give an obvious fluorescent signal change from red to blue based on the ICT process. The possible mechanism for this electrophilic addition process was confirmed using electrospray ionization mass spectrometry (ESI-MS) and1H-NMR spectra.DMANIexhibited a good selectivity, a large Stokes shift (248 nm), a low detection limit (40 nM), and a rapid response (within 20 s) to ONOO?. More importantly,DMANIwas successfully used for ratiometric imaging and monitoring of the fluctuations of mitochondrial ONOO?in living cells.
A Fluorescent Kinase Inhibitor that Exhibits Diagnostic Changes in Emission upon Binding
Fleming, Cassandra L.,Sandoz, Patrick A.,Inghardt, Tord,?nfelt, Bj?rn,Gr?tli, Morten,Andréasson, Joakim
supporting information, p. 15000 - 15004 (2019/09/17)
The development of a fluorescent LCK inhibitor that exhibits favourable solvatochromic properties upon binding the kinase is described. Fluorescent properties were realised through the inclusion of a prodan-derived fluorophore into the pharmacophore of an ATP-competitive kinase inhibitor. Fluorescence titration experiments demonstrate the solvatochromic properties of the inhibitor, in which dramatic increase in emission intensity and hypsochromic shift in emission maxima are clearly observed upon binding LCK. Microscopy experiments in cellular contexts together with flow cytometry show that the fluorescence intensity of the inhibitor correlates with the LCK concentration. Furthermore, multiphoton microscopy experiments demonstrate both the rapid cellular uptake of the inhibitor and that the two-photon cross section of the inhibitor is amenable for excitation at 700 nm.