52235-17-3

Basic information

• Product Name: Z-D-LEU-ONP
• Synonyms: Z-D-LEU-ONP;Z-D-LEUCINE-ONP;Z-D-LEUCINE 4-NITROPHENYL ESTER;BENZYLOXYCARBONYL-D-LEUCINE P-NITROPHENYL ESTER;BENZYLOXYCARBONYL-D-LEUCINE 4-NITROPHENYL ESTER;N-alpha-Benzyloxycarbonyl-D-leucine p-nitrophenyl ester;(R)-Z-2-amino-4-methyl-pentanoic acid 4-nitrophenyl ester;Z-D-leucine 4-nitrophenyl ester≥ 99% (HPLC)
• CAS NO: 52235-17-3
• Molecular Formula: C₂₀H₂₂N₂O₆
• Molecular Weight: 386.4
• Product Categories: Amino Acid Derivatives
• Mol File: 52235-17-3.mol
• Article Data: 1

Chemical Properties

• Boiling Point: 561.3±45.0 °C(Predicted)
• Appearance: /
• Density: 1.239±0.06 g/cm3(Predicted)
• Storage Temp.: -15°C
• PKA: 10.66±0.46(Predicted)
• CAS DataBase Reference: Z-D-LEU-ONP(CAS DataBase Reference)
• NIST Chemistry Reference: Z-D-LEU-ONP(52235-17-3)
• EPA Substance Registry System: Z-D-LEU-ONP(52235-17-3)

Safety Data

• Hazardous Substances Data: 52235-17-3(Hazardous Substances Data)

Usage

General Description

Z-D-LEU-ONP is a specific chemical compound that consists of Z-D-leucine bound to ortho-nitrophenyl (ONP) through a peptide bond. Z-D-leucine is a modified form of the amino acid leucine, where the amino group is protected with a benzoyl group (Z) to prevent unwanted reactions during peptide synthesis. The addition of ONP provides a chromophoric and fluorescent tag to the compound, making it useful for studying enzymatic reactions and protein interactions. Z-D-LEU-ONP is often used as a substrate for peptidases and proteases in biochemistry research and drug development, providing valuable insights into enzyme kinetics and inhibitor screening.

Downstream Products

• 294625-15-3 N-[3-(tert-butoxycarbonyl)aminopropyl]-2(R)-(carbobenzoxy)amino-4-methylpentanamide 
• 294625-17-5 N-[5-(tert-butoxycarbonyl)aminopentyl]-2(R)-(carbobenzoxy)amino-4-methylpentanamide 

Relevant articles and documents

Specificity of -chymotrypsin. The -chymotrypsin-catalyzed hydrolysis of N-acylamino acid p-nitrophenyl esters.

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