54249-88-6

Basic information

• Product Name: DIPEPTIDYLPEPTIDASE IV
• Synonyms: EC 3.4.14.5;DPP-IV;DPPIV (HUMAN);DIPEPTIDYLPEPTIDASE IV;DIPEPTIDYLPEPTIDASE IV, ASPERGILLUS ORIZAE;DIPEPTIDYL PEPTIDASE IV (HUMAN);DIPEPTIDYL AMINOPEPTIDASE IV;DIPEPTIDYL PEPTIDASE
• CAS NO: 54249-88-6
• Molecular Formula: NULL
• Molecular Weight: 0
• Mol File: 54249-88-6.mol
• Article Data: 0

Chemical Properties

• Appearance: /lyophilized powder
• Storage Temp.: −20°C
• Solubility: 0.1 M Tris-HCl, pH 8.0: soluble1 vial/mL, clear, colorless
• CAS DataBase Reference: DIPEPTIDYLPEPTIDASE IV(CAS DataBase Reference)
• NIST Chemistry Reference: DIPEPTIDYLPEPTIDASE IV(54249-88-6)
• EPA Substance Registry System: DIPEPTIDYLPEPTIDASE IV(54249-88-6)

Safety Data

• Hazard Codes: Xi,Xn,T
• Statements: 36/37/38-22-61
• Safety Statements: 26-36-45-53
• WGK Germany: 3
• Hazardous Substances Data: 54249-88-6(Hazardous Substances Data)

Usage

Description

Dipeptidyl Peptidase IV (DPP4) is an enzyme that is mapped to human chromosome 2q24.2 and is characterized by its N-glycosylation sites located in the β-propeller domain. It plays a crucial role in various biological processes and has been the subject of numerous studies due to its potential applications in different fields.

Uses

Used in Pharmaceutical Industry:
DPP4 is used as a therapeutic target for the development of drugs that inhibit its activity. This is because DPP4 inhibition has been shown to have beneficial effects on various health conditions, such as type 2 diabetes and cardiovascular diseases. By targeting DPP4, researchers aim to develop novel treatments that can improve patient outcomes and address unmet medical needs.
Used in Research and Development:
DPP4 is used as a subject of study in research and development to better understand its role in various biological processes and to identify potential applications in the field of medicine. For instance, a study was conducted to assess the interactive hemodynamic effects of DPP4 inhibition as well as the angiotensin-converting enzyme inhibition. This research helps to expand the knowledge base on DPP4 and its potential implications in human health.
Used in Allergen Characterization:
DPP4 has also been used in a study to identify and characterize the 100 kDa High Molecular Weight Hymenoptera Venom Allergens Api m 5 and Ves v 3. This application is particularly relevant in the field of allergy research, as understanding the structure and function of allergens can lead to the development of more effective diagnostic tools and treatment strategies for individuals with allergies.

Biochem/physiol Actions

Native DPPIV is a ubiquitous type II transmembrane glycoprotein and a serine protease of the S9 prolyl-oligopeptidase family. In vivo, it is synthesized with a signal peptide, which functions as the membrane anchoring domain. There is an 88% sequence homology between the human and porcine kidney enzymes. Both exist as homodimers with a subunit molecular weight of ~30 kDa. The high mannose 100 kDa DPPIV precursor is processed in the Golgi to yield a 124 kDa heavily N-and O-linked mature glycoprotein. It is then sorted to the apical membrane through the concerted action of both N- and O-linked glycans and its association with lipid microdomains. The porcine enzyme contains 18.3% carbohydrates, which the glycan composition is 0.9% fucose, 3.4% mannose, 5.1% galactose, 8.2% glucosamine, and 0.7% sialic acid. DPPIV is highly expressed on endothelial cells, epithelial cells, and lymphocytes. It is also present in plasma in its soluble form.

Purification Methods

The aminopeptidase is purified about 2000-fold by column chromatography on CM-cellulose, hydroxylapatite and Gly-Pro AH-Sepharose. [Imai et al. J Biochem (Tokyo) 93 431 1983, Schomburg & Schomburg Springer Handbook of Enzymes 2nd Edn vol 6 p 286 2002.] DNA (deoxyribonucleic acids). The essential structures of chromosomes are DNA and contain the genetic “blueprint” in the form of separate genes. They are made up of the four deoxyribonucleic acids (nucleotides): adenylic acid, guanylic acid, cytidylic acid and thymidylic acid (designated A, G, C, T respectively) linked together by their phosphate groups in ester bonds between the 3' and 5' hydroxy groups of the 2'-deoxy-D-ribose moiety of the nucleotides. The chains form a double-stranded spiral (helix) in which the two identical nucleotide sequences run antiparallel with the heterocyclic bases hydrogen bonded (A….T, G….C) forming the “ladder” between the strands. Short sequences of DNA are available commercially, are commercially custom made or synthesised in a DNA synthesiser and purified by HPLC. Their purity can be checked by restriction enzyme cleavage followed by gel electophoresis, or directly by gel electrophoresis or analytical HPLC. Commercial DNAs are usually pure enough for direct use but can be further purified using commercially available kits involving binding to silica or other matrices and eluting with Tris buffers. There are now rapid “throughput” techniques for sequencing DNA which are very accurate.