55019-72-2Relevant articles and documents
Efficient Assay for the Detection of Hydrogen Peroxide by Estimating Enzyme Promiscuous Activity in the Perhydrolysis Reaction
Wilk, Monika,Ostaszewski, Ryszard
, p. 1464 - 1469 (2021/02/01)
Hydrogen peroxide is an ideal oxidant in view of its availability, atom economy, or green aspects. Furthermore, it is produced by the cell mitochondria and plays a meaningful role in controlling physiological processes, but its unregulated production leads to the destruction of organs. Due to its diverse roles, a fast and selective method for hydrogen peroxide detection is the major limitation to preventing the negative effects caused by its excess. Therefore, we aimed to develop an efficient assay for the detection of H2O2. For this purpose, we combined the enzymatic method for the detection of hydrogen peroxide with the estimation of the promiscuity of various enzymes. We estimated the activity of an enzyme in the reaction of p-nitrophenyl esters with hydrogen peroxide resulting in the formation of peracid. To our knowledge, there is no example of a simple, multi-sensor demonstrating the promiscuous activity of an enzyme and detecting hydrogen peroxide in aqueous media.
Quick E. A Fast Spectrophotometric Method to Measure the Enantioselectivity of Hydrolases
Janes, Lana E.,Kazlauskas, Romas J.
, p. 4560 - 4561 (2007/10/03)
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Enantioselectivity Effects in Hydrolytic Cleavage of Activated Substrates with α- and β-Cyclodextrins
Fornasier, Roberto,Reniero, Fabiano,Scrimin, Paolo,Tonellato, Umberto
, p. 193 - 196 (2007/10/02)
The kinetics of hydrolytic cleavage of the enantiomers of p- and m-nitrophenyl (Np) phenylacatate esters of general structure PhCR1R2-CO2-Np where R1,R2=H, Me , H, OMe , or CF3, OMe , and of the carbonates PhCHMe-OCO2-p-Np (7) and n-C6H13-CHMe-OCO2-p-Np (8), have been measured in the presence of α- and β-cyclodextrins.The rates of intracomplex cleavage were found to be larger for the R- than for the S-enantiomers in almost all cases; the enantioselectivity factors range from unity to ca. 19 depending on the relative size of the pairs of substituents R1 and R2.The effects have been interpreted with the help of molecular models.