552-74-9Relevant articles and documents
Access to both anomers of rutinosyl azide using wild-type rutinosidase and its catalytic nucleophile mutant
Bojarová, Pavla,Brodsky, Katerina,Halada, Petr,Jav?rková, Hana,K?en, Vladimír,Konvalinková, Dorota,Kotik, Michael,Pelantová, Helena
, (2020/10/20)
Rutinosidases hydrolyze β-rutinosylated flavonoids. As retaining glycosidases they also have a transglycosylation activity. Here we show that two newly identified wild-type rutinosidases, which are members of the glycoside hydrolase family 5–23, are capable of glycosylation of an inorganic azide with rutin as a glycosyl donor, yielding rutinosyl β-azide. On the other hand, rutinosyl α-azide was synthesized by the catalytic nucleophile mutant of the rutinosidase from Aspergillus niger, which also belongs to GH5–23. Thus, we were able to synthesize at a preparatory scale both anomers of rutinosyl azide from rutin using either wild-type or mutant rutinosidases of GH5–23.
New assay of α-l-rhamnosidase
Karni?ová Potocká, Elena,Mastihubová, Mária,?i?ová, Iveta,Mastihuba, Vladimír
, p. 167 - 174 (2017/12/06)
Abstract: Free rutinose was prepared by enzymatic hydrolysis of rutin using defatted seed meal from tartary buckwheat. This disaccharide was used as substrate in spectrophotometric assay of α-l-rhamnosidase. The assay is based on hydrolysis of rutinose and subsequent determination of released glucose by a standard glucose oxidase assay kit. The method is easy to perform and requires no expensive equipment. The assay was applied in α-l-rhamnosidase estimation in ten commercial enzyme preparations and compared with standard assay on chromogenic substrate.
Dracopalmaside, a New Flavonoid from Dracocephalum palmatum
Olennikov,Chirikova
, p. 1067 - 1069 (2016/02/18)
Phytochemical studies of the aerial part of Dracocephalum palmatum (Lamiaceae) isolated the new flavonoid dracopalmaside that was identified based on UV, MS, and NMR spectral data as luteolin-7,4′-di-O-α -Lrhamnopyranosyl-(1→6)-β-D-glucopyranoside (luteolin-7,4′-di-O-rutinoside) and the two known compounds cynarotriside and luteolin-7,4′-di-O-glucoside.