56361-75-2Relevant articles and documents
Biosynthetic reconstitution of deoxysugar phosphoramidate metalloprotease inhibitors using an N-P-bond-forming kinase
Baulig, Alexandra,Helmle, Irina,Bader, Marius,Wolf, Felix,Kulik, Andreas,Al-Dilaimi, Arwa,Wibberg, Daniel,Kalinowski, J?rn,Gross, Harald,Kaysser, Leonard
, p. 4486 - 4490 (2019/04/29)
Phosphoramidon is a potent metalloprotease inhibitor and a widespread tool in cell biology research. It contains a dipeptide backbone that is uniquely linked to a 6-deoxysugar via a phosphoramidate bridge. Herein, we report the identification of a gene cluster for the formation of phosphoramidon and its detailed characterization. In vitro reconstitution of the biosynthesis established TalE as a phosphoramidate-forming kinase and TalC as the glycosyltransferase which installs the l-rhamnose moiety by phosphoester linkage.