5725-89-3 Usage
Description
Methoxyresorufin is a fluorogenic substrate for the cytochrome P450 (CYP) isoforms CYP1A1 and CYP1A2. Methyoxyresorufin is O-demethylated by CYP1A1/2, releasing resorufin, which displays excitation/emission maxima of 570/580 nm, respectively. The appearance of resorufin fluorescence can be used to quantify CYP1A1/2 activity.
Uses
Different sources of media describe the Uses of 5725-89-3 differently. You can refer to the following data:
1. Used to differentiate isozymes of cytochrome P-450
2. Substrate for Cytochrome P-450. METHOXYRESORUFIN is used for various investigations including the differentiatiation of cytochrome P-450 isozymes.
3. Resorufin methyl ether have been used as substrate in the incubation mixture, during the determination of cytochrome P4501A activities such as ethoxyresorufin O-deethylase (EROD) and methoxyresorufin O-demethylase(MROD) in liver microsomes, using high performance liquid chromatography(HPLC).
Biochem/physiol Actions
Fluorimetric substrate for cytochrome P450 linked enzymes.
Check Digit Verification of cas no
The CAS Registry Mumber 5725-89-3 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 5,7,2 and 5 respectively; the second part has 2 digits, 8 and 9 respectively.
Calculate Digit Verification of CAS Registry Number 5725-89:
(6*5)+(5*7)+(4*2)+(3*5)+(2*8)+(1*9)=113
113 % 10 = 3
So 5725-89-3 is a valid CAS Registry Number.
InChI:InChI=1/C13H9NO3/c1-16-9-3-5-11-13(7-9)17-12-6-8(15)2-4-10(12)14-11/h2-7H,1H3
5725-89-3Relevant articles and documents
Regioselective Difluoromethane sulfonylation and Triflylation of Resorufin Derivatives
Tan, Qingwei,Zhao, Shuxuan,Li, Yuyao,Jiang, Jialing,Tang, Huiling,Chen, Yefeng,Peng, Yan,Xie, Hexin
supporting information, p. 8477 - 8481 (2021/11/13)
Reported herein is a regioselective difluoromethane sulfonylation or triflylation of resorufin derivatives, which allows easy access to 2-difluoromethane sulfonylated or triflylated resorufin derivatives in good yields. The installation of a difluorometha
