58430-80-1Relevant articles and documents
Simple and easy synthesis of γ-amido-dNTPs in water and their polymerase reaction properties
Sakurada, Takato,Miyahara, Ryo,Kawazoe, Ryoji,Nagata, Yusuke,Kikukawa, Yoshiya,Sasaki, Shigeki,Taniguchi, Yosuke
, p. 1061 - 1061 (2021/11/20)
γ-Amido-modified 2′-deoxynucleoside triphosphates (dNTPs) and nucleoside triphosphates (NTPs) are becoming increasingly important as biological tools. We herein describe the simple and easy synthesis of γ-amido-dNTPs and -NTPs from commercially available corresponding dNTPs and NTPs in a one-pot reaction using water-soluble carbodiimide and ammonia solution. We examined the effects of synthesized γ-amido-dNTPs on the DNA polymerase reaction. The results obtained showed the incorporation of these derivatives into the DNA primer while maintaining nucleobase selectivity; however, their incorporation efficiency by DNA polymerase was lower than that of dNTP. This is the first study to demonstrate the successful synthesis of four sets of γ-amido-dNTPs and clarify their properties.
