58467-94-0Relevant articles and documents
Unsymmetrical pentamethine cyanines for visualizing physiological acidities from the whole-animal to the cellular scale with pH-responsive deep-red fluorescence
Cao, Chong,Du, Ling,Han, Limei,Hu, Jiayi,Lei, Zuhai,Li, Cong,Wang, Cong,Wang, Yicheng
, p. 17871 - 17879 (2021/05/29)
Acidity plays an important role in numerous physiological and pathological events. Non-invasively monitoring pH dynamics would be valuable for understanding pathological processes and optimizing therapeutic strategies. Although numerous near-infrared (NIR) fluorophores have been developed to monitor acidification in vivo, the experimental results are difficult to verify at the molecular or cellular level using a fluorescence microscope or flow cytometer due to the lack of lasers with excitation wavelengths in the NIR wavelength range. This work presents a sequential condensation strategy for obtaining unsymmetrical pentamethine cyanines with fine-tuned pKa values and improved yields. These deep-red fluorophores with pH responsiveness can not only be used to monitor acidification in live cells using confocal microscopic imaging and flow cytometry, but they can also be used to non-invasively identify infected tissue with a low pH value in live mouse models. In addition, the acidity in infected tissue slices was verified under a conventional confocal microscope. Overall, this work demonstrates a new synthetic method with improved yields for unsymmetrical pentamethine cyanines that can report acidity. These pH-responsive deep-red fluorophores not only provide new tools for accessing pH-associated physiological and pathological events, but they can also help in understanding in vivo imaging results at the molecular or cellular level due to their detectability by multiple imaging instruments.
Ratiometric fluorescent probes for selective and sensitive visualization of bacterial microenvironment protease activity
Wang, Qinghua,Wang, Xiang,Sun, Ying,Yang, Xiaoxiao,Zhang, Leilei,Zhang, Qingyang,Hu, Zhi-Qiang,Hu, Hai-Yu
supporting information, p. 5064 - 5067 (2019/05/06)
The extracellular SspA protease of the human pathogen S. aureus is essential for evading the host immune defence system. Two novel FRET probes for selective real-time ratiometric imaging of SspA activity in live S. aureus cells are reported.
Easily-modified near-infrared region II organic small molecule dye and synthetic method and application thereof
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Paragraph 0069-0072; 0077, (2019/03/28)
The invention provides an easily-modified near-infrared region II organic small molecule dye and a synthetic method and application of the easily-modified near-infrared region II organic small molecule dye. The near-infrared region II fluorescent dye belongs to polymethenyl pyran or polymethenyl thiapyran salt organic small molecules, and is prepared by a condensation reaction of polymethenyl aniline salt with pyran or thiopyran salt. The synthetic raw materials are easy to obtain, the cost is low, the preparation process is simple, the yield is high, and large-amount synthesis can be achieved. The purpose of adjusting the fluorescence emission spectrum is achieved by changing an intramolecular polymethenyl chain and hetero atom species. The small molecule dye has the maximum emission wavelength range of 1000-1200 nm, and is high in fluorescence quantum yield, good in light stability and quite applicable to living imaging. The small molecule dye can be linked to groups with specific functions by click chemistry for a variety of bioimaging application. Near-infrared region II fluorescent living imaging achieves low background noise, strong fluorescence signal and high signal-to-noise ratio.
