58695-41-3Relevant articles and documents
2-Mercapto-Quinazolinones as Inhibitors of Type II NADH Dehydrogenase and Mycobacterium tuberculosis: Structure-Activity Relationships, Mechanism of Action and Absorption, Distribution, Metabolism, and Excretion Characterization
Murugesan, Dinakaran,Ray, Peter C.,Bayliss, Tracy,Prosser, Gareth A.,Harrison, Justin R.,Green, Kirsteen,Soares De Melo, Candice,Feng, Tzu-Shean,Street, Leslie J.,Chibale, Kelly,Warner, Digby F.,Mizrahi, Valerie,Epemolu, Ola,Scullion, Paul,Ellis, Lucy,Riley, Jennifer,Shishikura, Yoko,Ferguson, Liam,Osuna-Cabello, Maria,Read, Kevin D.,Green, Simon R.,Lamprecht, Dirk A.,Steyn, Adrie J. C.,Ioerger, Thomas R.,Sacchettini, Jim,Rhee, Kyu Y.,Arora, Kriti,Barry, Clifton E.,Wyatt, Paul G.,Boshoff, Helena I. M.
, p. 954 - 969 (2018/06/14)
Mycobacterium tuberculosis (MTb) possesses two nonproton pumping type II NADH dehydrogenase (NDH-2) enzymes which are predicted to be jointly essential for respiratory metabolism. Furthermore, the structure of a closely related bacterial NDH-2 has been reported recently, allowing for the structure-based design of small-molecule inhibitors. Herein, we disclose MTb whole-cell structure-activity relationships (SARs) for a series of 2-mercapto-quinazolinones which target the ndh encoded NDH-2 with nanomolar potencies. The compounds were inactivated by glutathione-dependent adduct formation as well as quinazolinone oxidation in microsomes. Pharmacokinetic studies demonstrated modest bioavailability and compound exposures. Resistance to the compounds in MTb was conferred by promoter mutations in the alternative nonessential NDH-2 encoded by ndhA in MTb. Bioenergetic analyses revealed a decrease in oxygen consumption rates in response to inhibitor in cells in which membrane potential was uncoupled from ATP production, while inverted membrane vesicles showed mercapto-quinazolinone-dependent inhibition of ATP production when NADH was the electron donor to the respiratory chain. Enzyme kinetic studies further demonstrated noncompetitive inhibition, suggesting binding of this scaffold to an allosteric site. In summary, while the initial MTb SAR showed limited improvement in potency, these results, combined with structural information on the bacterial protein, will aid in the future discovery of new and improved NDH-2 inhibitors.
ANTITHROMBOTIC AGENTS
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, (2008/06/13)
This invention relates to thrombin inhibiting compounds having the Formula IX--Y--NH--(CH 2) r--G I where X, Y, r and G have the values defined in the description, as well as pharmaceutical formulations containing those compounds and methods of their use as thrombin inhibitors, coagulation inhibitors, and thromboembolic disorder agents.
Mesoionic compounds. 10. Preferred conformation of a 3-alkyl and 3-aryl-substituent in 4-unsubstituted and 4-acylated monocyclic munchnones and sydnones
Petride, Horia,Cort, Antonella Dalla,Florea, Cristina,Cǎproiu, Miron
, p. 249 - 259 (2007/10/03)
A new 4-unsubstituted monocyclic oxazolium-5-olate (7c) was generated and NMR-characterized. Several 3-alkyl- and 3-aryl-4-acylated monocyclic munchnone and sydnone derivatives were also prepared. From NMR experimental data and theoretical calculations di
