59837-14-8 Usage
Description
P-NITROPHENYL 2-ACETAMIDO-2-DEOXY-3-O-(BETA-D-GALACTOPYRANOSYL)-ALPHA-D-GALACTOPYRANOSIDE is a complex organic compound that serves as a substrate for various enzymes and is used in the study and detection of specific biochemical processes. It is a white crystalline solid with unique chemical properties that make it valuable in the field of biochemistry and molecular biology.
Uses
Used in Biochemical Research:
P-NITROPHENYL 2-ACETAMIDO-2-DEOXY-3-O-(BETA-D-GALACTOPYRANOSYL)-ALPHA-D-GALACTOPYRANOSIDE is used as a substrate for Acetylgalactosaminidase, an enzyme involved in the metabolism of glycoproteins and glycolipids. This application is crucial for studying the enzyme's function and its role in various biological processes.
Used in Microbial Analysis:
In the field of microbiology, P-NITROPHENYL 2-ACETAMIDO-2-DEOXY-3-O-(BETA-D-GALACTOPYRANOSYL)-ALPHA-D-GALACTOPYRANOSIDE is used as a substrate for Clostridium and Bifidobacterium species. This allows researchers to identify and differentiate these bacteria based on their ability to metabolize the compound.
Used in Enzyme Assays:
P-NITROPHENYL 2-ACETAMIDO-2-DEOXY-3-O-(BETA-D-GALACTOPYRANOSYL)-ALPHA-D-GALACTOPYRANOSIDE is used as a fluorescent substrate for α2,3-(O)-Sialyltransferase, an enzyme that plays a role in the synthesis of sialic acid-containing glycoconjugates. This application is essential for understanding the enzyme's activity and its involvement in various biological pathways.
Used in Pharmaceutical Development:
Due to its role as a substrate for specific enzymes, P-NITROPHENYL 2-ACETAMIDO-2-DEOXY-3-O-(BETA-D-GALACTOPYRANOSYL)-ALPHA-D-GALACTOPYRANOSIDE may have potential applications in the development of drugs targeting these enzymes, particularly in the treatment of diseases related to their dysfunction.
Used in Diagnostics:
P-NITROPHENYL 2-ACETAMIDO-2-DEOXY-3-O-(BETA-D-GALACTOPYRANOSYL)-ALPHA-D-GALACTOPYRANOSIDE's ability to serve as a substrate for various enzymes makes it a valuable tool in the development of diagnostic tests to detect and monitor enzyme activity, which can be indicative of certain health conditions or responses to treatment.
Check Digit Verification of cas no
The CAS Registry Mumber 59837-14-8 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 5,9,8,3 and 7 respectively; the second part has 2 digits, 1 and 4 respectively.
Calculate Digit Verification of CAS Registry Number 59837-14:
(7*5)+(6*9)+(5*8)+(4*3)+(3*7)+(2*1)+(1*4)=168
168 % 10 = 8
So 59837-14-8 is a valid CAS Registry Number.
InChI:InChI=1/C20H28N2O13/c1-8(25)21-13-18(35-20-17(29)16(28)14(26)11(6-23)34-20)15(27)12(7-24)33-19(13)32-10-4-2-9(3-5-10)22(30)31/h2-5,11-20,23-24,26-29H,6-7H2,1H3,(H,21,25)/t11-,12-,13-,14+,15+,16+,17-,18?,19+,20+/m1/s1
59837-14-8Relevant articles and documents
Characterization of a galactosynthase derived from bacillus circulans β-galactosidase: Facile synthesis of D -lacto- and D -galacto-N-bioside
Li, Chao,Kim, Young-Wan
, p. 522 - 526 (2014/03/21)
Glycosynthases - retaining glycosidases mutated at their catalytic nucleophile - catalyze the formation of glycosidic bonds from glycosyl fluorides as donor sugars and various glycosides as acceptor sugars. Here the first glycosynthase derived from a family 35 β-galactosidase is described. The Glu→Gly mutant of BgaC from Bacillus circulans (BgaC-E233G) catalyzed regioselective galactosylation at the 3-position of the sugar acceptors with α-galactosyl fluoride as the donor. Transfer to 4-nitophenyl α-D-N-acetyl-glucosaminide and α-D-N-acetylgalactosaminide yielded 4-nitophenyl α-lacto-N-biose and α-galacto-N-biose, respectively, in high yields (up to 98 %). Kinetic analysis revealed that the high affinity of the acceptors contributed mostly to the BgaC-E233G-catalyzed transglycosylation. BgaC-E233G showed no activity with β-(1,3)-linked disaccharides as acceptors, thus suggesting that this enzyme can be used in one-pot synthesis of LNB- or GNB-containing glycans. The first glycosynthase from family 35 β-galactosidase: A β-galactosidase from Bacillus circulans (BgaC) was converted into a glycosynthase that catalyzes regioselective galactosylation at the 3-OH group of sugar acceptors. The yields were almost quantitative: up to 98 % lacto-N-bioside and galacto-N-bioside were obtained.
Regioselective synthesis of p-nitrophenyl glycosides of β-D-galactopyranosyl-disaccharides by transglycosylation with β-D-galactosidases
Zeng, Xiaoxiong,Yoshino, Rika,Murata, Takeomi,Ajisaka, Katsumi,Usui, Taichi
, p. 120 - 131 (2007/10/03)
The β-D-galactosidase from porcine liver induced regiospecific transglycosylation of β-D-galactose from β-D-Gal-OC6H4NO2-o to OH-6 of, respectively, p-nitrophenyl glycoside acceptors of Gal, GlcNAc and GalNAc to afford β-G
