6154-45-6 Usage
Description
BZ-TYR-PNA, also known as N-Benzoyl-L-tyrosine p-nitroanilide, is a synthetic compound commonly utilized in the field of biochemistry and molecular biology. It possesses unique structural properties that allow it to interact with specific enzymes, making it a valuable tool for research and diagnostic purposes.
Uses
Used in Biochemical Research:
BZ-TYR-PNA is used as a substrate for identifying, differentiating, and characterizing serine carboxypeptidases and various proteases. Its interaction with these enzymes helps researchers understand their structure, function, and potential roles in biological processes.
Used in Diagnostic Applications:
In the field of diagnostics, BZ-TYR-PNA serves as a valuable tool for detecting and monitoring the activity of specific enzymes. By measuring the rate of hydrolysis of BZ-TYR-PNA by these enzymes, clinicians can gain insights into the presence and severity of certain conditions or diseases.
Used in Drug Development:
BZ-TYR-PNA also plays a crucial role in the development of new drugs targeting serine carboxypeptidases and proteases. By using this compound as a starting point, researchers can design and synthesize novel inhibitors or activators that modulate the activity of these enzymes, potentially leading to the development of new therapeutic agents for various diseases.
Used in Quality Control:
In the pharmaceutical industry, BZ-TYR-PNA is employed as a quality control standard for the assessment of enzyme activity in drug formulations. By comparing the activity of enzymes in a drug product to that of a known concentration of BZ-TYR-PNA, manufacturers can ensure the consistency and efficacy of their products.
Check Digit Verification of cas no
The CAS Registry Mumber 6154-45-6 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 6,1,5 and 4 respectively; the second part has 2 digits, 4 and 5 respectively.
Calculate Digit Verification of CAS Registry Number 6154-45:
(6*6)+(5*1)+(4*5)+(3*4)+(2*4)+(1*5)=86
86 % 10 = 6
So 6154-45-6 is a valid CAS Registry Number.
InChI:InChI=1/C22H19N3O5/c26-19-12-6-15(7-13-19)14-20(24-21(27)16-4-2-1-3-5-16)22(28)23-17-8-10-18(11-9-17)25(29)30/h1-13,20,26H,14H2,(H,23,28)(H,24,27)
6154-45-6Relevant articles and documents
Acid-Sensitive Latent Inhibitors for Proteolytic Enzymes: Synthesis and Characterization
Silver, Marc S.,Haskell, John H.
, p. 1253 - 1259 (2007/10/02)
The reaction between peptide aldehydes and acylhydrazones affords derivatives that represent potential prodrugs for selective inhibition of lysosomal enzymes.BzPheal=Ala, obtained from the reaction between N-benzoyl-L-phenylalaninal and N-acetyl-L-alanine hydrazide, has been most carefully studied.When BzPheal=Ala is introduced into ongoing reactions catalyzed by α-chymotrypsin or papain, the rate of these reactions diminishes more rapidly with time than do those of controls lacking BzPheal=Ala.Furthermore, the disparity between run and control is much greater at pH 5 than at pH 7.The extent of inhibition (defined as explained in the text) at pH 5 can exceed that at pH 7 by 25-40-fold.The data are quantitatively explained by a reaction scheme that recognizes three important properties of BzPheal=Ala: (1) It undergoes hydrolysis at pH 5-7 to regenerate N-benzoyl-L-phenylalaninal; (2) the aldehyde thus liberated is a far more potent inhibitor for serine or cysteine proteases than is BzPheal=Ala; and (3) the rate constant for hydrolysis of BzPheal=Ala at pH 5 greatly exceeds that at pH 7.
