6342-17-2 Usage
Description
1,4-Diacryloylpiperazine (PIP) is a reagent that can be substituted for bis-acrylamide in the preparation of polyacrylamide gels. It is an off-white solid with chemical properties that make it suitable for various applications in the field of biochemistry and molecular biology.
Uses
Used in Biochemistry and Molecular Biology:
1,4-Diacryloylpiperazine is used as a crosslinker in polyacrylamide gels for enhancing their physical strength and improving the separation and detection of proteins. This application is particularly useful in techniques such as polyacrylamide gel electrophoresis (PAGE), isoelectric focusing (IEF), 2D electrophoresis, and protein sequencing.
Used in Polyacrylamide Gel Electrophoresis (PAGE):
1,4-Diacryloylpiperazine is used as a crosslinker in polyacrylamide gels for PAGE, providing increased physical strength and improved separation and detection of proteins. This allows for more accurate and efficient analysis of protein samples.
Used in Isoelectric Focusing (IEF):
In IEF, 1,4-Diacryloylpiperazine is used as a crosslinker in polyacrylamide gels to improve the separation of proteins based on their isoelectric points, leading to better resolution and identification of individual proteins.
Used in 2D Electrophoresis:
1,4-Diacryloylpiperazine is used as a crosslinker in the second dimension of 2D electrophoresis, where it helps in the improved separation and detection of proteins after their initial separation based on isoelectric points in the first dimension.
Used in Protein Sequencing:
In protein sequencing, 1,4-Diacryloylpiperazine is used as a crosslinker in polyacrylamide gels to enhance the separation of peptide fragments, allowing for more accurate determination of protein sequences.
Check Digit Verification of cas no
The CAS Registry Mumber 6342-17-2 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 6,3,4 and 2 respectively; the second part has 2 digits, 1 and 7 respectively.
Calculate Digit Verification of CAS Registry Number 6342-17:
(6*6)+(5*3)+(4*4)+(3*2)+(2*1)+(1*7)=82
82 % 10 = 2
So 6342-17-2 is a valid CAS Registry Number.
InChI:InChI=1/C10H14N2O2/c1-3-9(13)11-5-7-12(8-6-11)10(14)4-2/h3-4H,1-2,5-8H2
6342-17-2Relevant articles and documents
COMPOSES CETONIQUES SUPPORTES CATALYSEURS D'HYDRATATION DES α-AMINONITRILES
Sola, R.,Brugidou, J.,Taillades, J.,Commeyras, A.,Previero, A.
, p. 1501 - 1504 (1983)
The preparation of hydrophilic crosslinked polymers which are carrying carbonyl groups of the 4-piperidone type is described.These compounds catalyse the hydration of α-aminonitriles to α-aminoamides.The main parameters involved in the catalytic activity are studied.
Preparation method of acrylamide-based compounds
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Paragraph 0017, (2018/01/11)
The present invention relates to the field of fine chemical materials, particularly to a new mild, efficient and economical preparation process technology of a class of acrylamide-type compounds, wherein a beta-amine substituted propionamide precursor is subjected to an in-situ amine elimination reaction under the action of a suitable electrophilic reagent so as to prepare the target product.
Total synthesis of amiclenomycin, an inhibitor of biotin biosynthesis.
Mann, Stephane,Carillon, Sophie,Breyne, Olivier,Marquet, Andree
, p. 439 - 450 (2007/10/03)
We describe the first synthesis of amiclenomycin, a natural product that has been found to inhibit biotin biosynthesis and, as a consequence, to exhibit antibiotic properties. Structure 1, with a trans relationship between the ring substituents. had previously been proposed for amiclenomycin on the basis of its 1H NMR spectrum. We have prepared the trans and cis isomers 1 and 2 by unequivocal routes and we conclude that the natural product is in fact the cis isomer 2. The properly substituted cyclohexadienyl rings were constructed first. A cycloaddition reaction between 1,2-di(phenylsulfonyl)ethylene and the N-allyloxycarbonyl diene 13, followed by reductive elimination of the phenylsulfinyl groups, gave the cis isomer 15. To obtain the trans isomer, the O-trimethylsilyl diene was used to give the cis hydroxylated Diels-Alder adduct 33, which was transformed into the corresponding trans amino derivative by means of a Mitsunobu reaction. The L-alpha-amino acid functionality was introduced by means of a Strecker reaction on the aldehydes 16 and 42, followed by enzymatic hydrolysis with immobilised pronase.
