63959-45-5

Basic information

• Product Name: SHIKIMATE-3-PHOSPHATE
• Synonyms: SHIKIMATE-3-PHOSPHATE;S-3-P;shikimic acid-3-phosphate;(-)-Phosphoric acid [(1R)-3-(carboxylato)-5β,6α-dihydroxy-2-cyclohexene-1α-yl] ester;Phosphoric acid [(3R)-4β,5α-dihydroxy-1-carboxylato-1-cyclohexene-3β-yl] ester;(3R,4S,5R)-;ShikiMate-3-phosphate DISCONTINUED. See S357026
• CAS NO: 63959-45-5
• Molecular Formula: C₇H₁₁O₈P
• Molecular Weight: 254.13
• Product Categories: Various Metabolites and Impurities;Metabolites;Phosphorylating and Phosphitylating Agents;Chiral Reagents
• Mol File: 63959-45-5.mol
• Article Data: 3

Chemical Properties

• Boiling Point: 585.8°Cat760mmHg
• Flash Point: 308.1°C
• Appearance: /solid
• Density: 1.84g/cm³
• Vapor Pressure: 3.67E-16mmHg at 25°C
• Refractive Index: 1.616
• Storage Temp.: Hygroscopic, Refrigerator, Under Inert Atmosphere
• Solubility: Water
• Stability: Very Hygroscopic, Moisture Sensitive, Store under Argon and in Freezer
• CAS DataBase Reference: SHIKIMATE-3-PHOSPHATE(CAS DataBase Reference)
• NIST Chemistry Reference: SHIKIMATE-3-PHOSPHATE(63959-45-5)
• EPA Substance Registry System: SHIKIMATE-3-PHOSPHATE(63959-45-5)

Safety Data

• WGK Germany: 3
• Hazardous Substances Data: 63959-45-5(Hazardous Substances Data)

Usage

Description

SHIKIMATE-3-PHOSPHATE, also known as S-3-P, is an essential intermediate in the shikimate pathway, a metabolic pathway responsible for the biosynthesis of aromatic amino acids and other secondary metabolites in plants, bacteria, and fungi. It plays a crucial role in the production of these vital compounds, which are necessary for the proper functioning and growth of these organisms.

Uses

Used in Agricultural Industry:
SHIKIMATE-3-PHOSPHATE is used as a target for the broad-spectrum herbicide N-(phosphonomethyl)glycine (glyphosate). Glyphosate inhibits the activity of 5-Enolpyruvoyl-shikimate 3-phosphate synthase (EPSPS), an enzyme that catalyzes a key step in the shikimate pathway. By targeting EPSPS, glyphosate effectively disrupts the production of essential aromatic amino acids in plants, leading to their eventual death. This makes glyphosate a widely used herbicide for controlling various types of weeds in agricultural settings.

InChI:InChI=1/C7H11O8P/c8-4-1-3(7(10)11)2-5(6(4)9)15-16(12,13)14/h2,4-6,8-9H,1H2,(H,10,11)(H2,12,13,14)/t4-,5-,6+/m1/s1

Upstream product

• 89771-75-5 5-enol-pyruvoylshikimate 3-phosphate (EPSP) 
• 138-59-0 shikimic acid 

Downstream Products

• 89771-75-5 5-enol-pyruvoylshikimate 3-phosphate (EPSP) 

Relevant articles and documents

Study of the Phosphoryl-Transfer Mechanism of Shikimate Kinase by NMR Spectroscopy

The phosphoryl-transfer mechanism of shikimate kinase from Mycobacterium tuberculosis and Helicobacter pylori, which is an attractive target for antibiotic drug discovery, has been studied by 1D 1H and 31P NMR spectroscopy. Metaphosphoric acid proved to be a good mimetic of the metaphosphate intermediate and facilitated the ready and rapid evaluation by NMR spectroscopic analysis of a dissociative mechanism. The required closed form of the active site for catalysis was achieved by the use of ADP (product) or two synthetic ADP analogues (AMPNP, AMPCP). Molecular dynamics simulation studies reported here also revealed that the essential arginine (Arg116/Arg117 in H. pylori and M. tuberculosis, respectively), which activates the γ-phosphate group of ATP for catalysis and triggers the release of the product for turnover, would also be involved in the stabilisation of the metaphosphate intermediate during catalysis. We believe that the studies reported here will be helpful for future structure-based design of inhibitors of this attractive target. The approach is also expected be useful for studies on the possible dissociative mechanism of other kinase enzymes.

Acid-catalyzed hydrolysis of 5-enolpyruvylshikimate 3-phosphate (EPSP) and some simple models of its vinyl ether functional group

Rates of hydrolysis of the vinyl ether functional groups of 5-enolpyruvylshikimate 3-phosphate (EPSP), α-methoxyacrylic acid, and methyl α-methoxyacrylate were measured in concentrated and dilute mineral acid solutions and carboxylic acid buffers, and rate profiles were constructed from the data so obtained. The results for methyl α-methoxyacrylate show a monotonic decrease in reaction rate with decreasing solvent acidity, culminating in a first-order dependence on [H+] in dilute acids; this and solvent isotope effects on this reaction indicate that this process occurs by the conventional mechanism for vinyl ether hydrolysis involving rate-determining proton transfer from hydronium ion to the substrate. The rate profiles for EPSP and α-methoxyacrylic acid are similar to that of methyl α-methoxyacrylate in concentrated acids, but they show uncatalyzed plateaus in the region pCH+ = 1-3 that then give way to renewed acid catalysis beyond pCH = 4. Solvent isotope effects suggest that these plateaus represent reaction via ionization of the carboxylic acid groups of these substrates followed by conventional hydrolysis of the vinyl ether groups of the carboxylate ions. Analysis of the data gives pKa = 3.77 for EPSP and pKa = 3.46 for α-methoxyacrylic acid and provides rate constants which show rate-retarding substituent effects on vinyl ether hydrolysis of 16 for α-CO2-, 20 000 for α-CO2H, and 81 000 for α-CO2Me.