64664-99-9

Basic information

• Product Name: CUG
• Synonyms: 3-CARBOXYUMBELLIFERYL BETA-D-GALACTOPYRANOSIDE;CARBOXYUMBELLIFERYL BETA-D-GALACTOPYRANOSIDE;CUG;3-CARBOXYUMBELLIFERYL B-D-GALACTOPYRANOS IDE;3-Carboxyumbelliferyl-D-galactopyranoside;Carboxyumbelliferyl--D-Galactopyranoside(CUG);3-Carboxyumbelliferyl β-D-galactopyranoside ,98%;CUG [3-CarboxyuMbelliferyl β-D-galactopyranoside]
• CAS NO: 64664-99-9
• Molecular Formula: C16H16O10
• Molecular Weight: 368.29
• EINECS: 1533716-785-6
• Mol File: 64664-99-9.mol
• Article Data: 0

Chemical Properties

• Melting Point: 186-190 °C(lit.)
• Boiling Point: 699.497oC at 760 mmHg
• Flash Point: 259.686oC
• Appearance: /
• Density: 1.718g/cm3
• Vapor Pressure: 0mmHg at 25°C
• Refractive Index: 1.686
• Storage Temp.: −20°C
• PKA: 1.92±0.20(Predicted)
• CAS DataBase Reference: CUG(CAS DataBase Reference)
• NIST Chemistry Reference: CUG(64664-99-9)
• EPA Substance Registry System: CUG(64664-99-9)

Safety Data

• Safety Statements: 22-24/25
• WGK Germany: 3
• F: 8-10-21
• Hazardous Substances Data: 64664-99-9(Hazardous Substances Data)

Usage

Description

3-Carboxyumbelliferyl beta-D-galactopyranoside is a synthetic substrate commonly used in biochemical assays. It is a chromogenic compound that, upon enzymatic cleavage by beta-galactosidase, releases a fluorescent product, 3-carboxyumbelliferone. This property makes it a valuable tool for detecting and quantifying beta-galactosidase activity in various biological samples.

Uses

Used in Research Applications:
3-Carboxyumbelliferyl beta-D-galactopyranoside is used as a research tool for detecting and quantifying beta-galactosidase enzyme activity. The release of the fluorescent product, 3-carboxyumbelliferone, upon enzymatic cleavage allows for the assessment of enzyme activity in various biological samples, such as cell lysates, tissue extracts, and purified enzyme preparations.
Used in Diagnostic Applications:
In the field of diagnostics, 3-Carboxyumbelliferyl beta-D-galactopyranoside serves as a substrate for the detection of lysosomal storage disorders, such as Gaucher's disease and Fabry disease. These conditions are characterized by a deficiency in specific lysosomal enzymes, including beta-galactosidase. By measuring the enzyme activity using this substrate, clinicians can diagnose and monitor the progression of these diseases.
Used in Pharmaceutical Development:
3-Carboxyumbelliferyl beta-D-galactopyranoside is also utilized in the development of new drugs targeting lysosomal storage disorders and other enzyme-related diseases. By using this substrate in high-throughput screening assays, researchers can identify potential drug candidates that modulate beta-galactosidase activity, leading to the development of novel therapeutic agents.
Used in Quality Control:
In the biotechnology and pharmaceutical industries, 3-Carboxyumbelliferyl beta-D-galactopyranoside is employed as a quality control tool to assess the purity and activity of beta-galactosidase enzyme preparations. By measuring the enzyme activity using this substrate, manufacturers can ensure the consistency and efficacy of their enzyme products.

InChI:InChI=1/C16H16O10/c17-5-10-11(18)12(19)13(20)16(26-10)24-7-2-1-6-3-8(14(21)22)15(23)25-9(6)4-7/h1-4,10-13,16-20H,5H2,(H,21,22)/t10-,11+,12+,13-,16-/m1/s1