64755-27-7

Basic information

• Product Name: 1-Formyl-2-dansylhydrazin
• CAS NO: 64755-27-7
• Molecular Weight: 293.346
• Mol File: 64755-27-7.mol
• Article Data: 1

Chemical Properties

• CAS DataBase Reference: 1-Formyl-2-dansylhydrazin(CAS DataBase Reference)
• NIST Chemistry Reference: 1-Formyl-2-dansylhydrazin(64755-27-7)
• EPA Substance Registry System: 1-Formyl-2-dansylhydrazin(64755-27-7)

Safety Data

• Hazardous Substances Data: 64755-27-7(Hazardous Substances Data)

Upstream product

• 605-65-2 5-(dimethylamino)naphth-1-ylsulfonyl chloride 
• 624-84-0 formic acid hydrazide 

Relevant articles and documents

Purification and characterization of animal porphobilinogen synthases. I. Bovine liver porphobilinogen synthase

Porphobilinogen synthase was purified from ox liver by ammonium sulfate fractionation, heat denaturation and column chromatography (purification: 400-fold; specific activity 4.72 nkat). The molecular weight of the native enzyme obtained by thin-layer gel filtration is about 280 000. Using 8M urea in the presence of dithiothreitol as reducing agent, the molecule breaks down into 8 subunits of molecular weight 36 000 (dodecyl-sulfate gel electrophoresis); the preparation of aminoethylated subunit is described. According to the above-mentioned molecular weight and to the quantitative amino acid analysis after total hydrolysis, the following composition of the enzyme sbunit was calculated Asx23-24 Thr7 Ser23-24 Glx29-31 Pro22-23 Gly22-24 Ala36-37 Val23-26 Met7 Ile9 Leu34-35 Tyr10 Phe11-12 Lys11-12 Cys6-7 His6-8 Arg22 Trp1-2. The subunits, having two free sulfhydryl groups, therefore consist of a chain of about 306 amino acids. The Dansyl-Edman procedure did not enable identification of any free N-terminal amino acid. The acyl group blocking the N-terminus is an acetyl group. It was identified, after hydrazinolysis of the enzyme, by means of chromatographic comparison with 1-formyl-2-dansyl-hydrazine and 1-acetyl-2-dansylhydrazine, whose syntheses and UV spectra are described.