69254-37-1 Usage
Description
5Z,8Z,11Z,14Z-EICOSATETRAENOIC-5,6,8,9,11,12,14,15-D8 ACID, also known as Arachidonic acid-d8, is a deuterated form of arachidonic acid containing eight deuterium atoms at the 5, 6, 8, 9, 11, 12, 14, and 15 positions. It is an essential fatty acid and serves as a precursor for all prostaglandins, thromboxanes, and leukotrienes. Arachidonic acid plays a crucial role in cell signaling and inflammation, as it is esterified in membrane phospholipids and tightly regulated through multiple interconnected pathways. The free form of arachidonic acid is a transient, critical substrate for the biosynthesis of eicosanoid second messengers.
Uses
Used in Mass Spectrometry Applications:
5Z,8Z,11Z,14Z-EICOSATETRAENOIC-5,6,8,9,11,12,14,15-D8 ACID is used as an internal standard for the quantification of arachidonic acid by gas chromatography (GC) or liquid chromatography (LC) mass spectrometry. This application is crucial for accurate measurement and analysis of arachidonic acid levels in various biological samples, such as blood, tissues, and cells.
Used in Pharmaceutical Industry:
In the pharmaceutical industry, 5Z,8Z,11Z,14Z-EICOSATETRAENOIC-5,6,8,9,11,12,14,15-D8 ACID is used as a research tool for the development of drugs targeting arachidonic acid metabolism and its related pathways. This helps in understanding the role of arachidonic acid in various diseases and conditions, such as inflammation, pain, and cardiovascular disorders.
Used in Nutritional Research:
5Z,8Z,11Z,14Z-EICOSATETRAENOIC-5,6,8,9,11,12,14,15-D8 ACID is utilized in nutritional research to study the role of arachidonic acid in human health and development. This aids in the development of dietary supplements and functional foods that can help maintain optimal health and prevent various diseases.
Used in Cell Signaling and Inflammation Studies:
In cell signaling and inflammation research, 5Z,8Z,11Z,14Z-EICOSATETRAENOIC-5,6,8,9,11,12,14,15-D8 ACID is employed to investigate the role of arachidonic acid in receptor-stimulated release, metabolism, and re-uptake of free arachidonate. This helps in understanding the complex mechanisms involved in cell signaling and inflammation, which can lead to the development of targeted therapies for various inflammatory conditions.
Check Digit Verification of cas no
The CAS Registry Mumber 69254-37-1 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 6,9,2,5 and 4 respectively; the second part has 2 digits, 3 and 7 respectively.
Calculate Digit Verification of CAS Registry Number 69254-37:
(7*6)+(6*9)+(5*2)+(4*5)+(3*4)+(2*3)+(1*7)=151
151 % 10 = 1
So 69254-37-1 is a valid CAS Registry Number.
69254-37-1Relevant articles and documents
Simultaneous determination of 2-arachidonoylglycerol, 1- arachidonoylglycerol and arachidonic acid in mouse brain tissue using liquid chromatography/tandem mass spectrometry
Zhang, Mei-Yi,Gao, Ying,Btesh, Joan,Kagan, Natasha,Kerns, Edward,Samad, Tarek A.,Chanda, Pranab K.
scheme or table, p. 167 - 177 (2010/08/06)
Endocannabinoids (ECs), such as anandamide (AEA) and 2-arachidonoylglycerol (2-AG), modulate a number of physiological processes, including pain, appetite and emotional state. Levels of ECs are tightly controlled by enzymatic biosynthesis and degradation in vivo. However, there is limited knowledge about the enzymes that terminate signaling of the major brain EC, 2-AG. Identification and quantification of 2-AG, 1-AG and arachidonic acid (AA) is important for studying the enzymatic hydrolysis of 2-AG. We have developed a sensitive and specific quantification method for simultaneous determination of 2-AG, 1-AG and AA from mouse brain and adipose tissues by liquid chromatography/tandem mass spectrometry (LC/MS/MS) using a simple brain sample preparationmethod. The separations were carried out based on reversed phase chromatography. Optimization of electrospray ionization conditions established the limits of detection (S/N=3) at 50, 25 and 65 fmol for 2-AG, 1-AG and AA, respectively. The methodswere selective, precise (%R.S.D.a range of 0.02-20, 0.01-10 and 0.05-50 ng/mg tissue for 2-AG, 1-AG and AA, respectively. The quantificationmethod was validated with consideration of thematrix effects and the mass spectrometry (MS) responses of the analytes and the deuterium labeled internal standard (IS). The developed methods were applied to study the hydrolysis of 2-AG from mouse brain extracts containingmembrane bound monoacylglycerol lipase (MAGL), and to measure the basal levels of 2-AG, 1-AG and AA in mouse brain and adipose tissues. Copyright
