73018-10-7Relevant articles and documents
Highly uniform self-assembled monolayers of silver nanospheres for the sensitive and quantitative detection of glutathione by SERS
Chen, Yi,Huang, Feixiang,Jiang, Cailing,Jiang, Li
supporting information, p. 10436 - 10445 (2021/08/09)
The homeostasis and imbalance of glutathione (GSH), an important antioxidant in organisms, are one of the key signals that reflect the health of organisms. In this paper, a novel SERS sensing platform based on Ag film@Si that self-assembled using silver nanospheres was proposed, which was used for the highly sensitive and selective detection of GSH. With the aid of an oil/water/oil three-phase system, the nano-silver film was self-assembled and finally deposited on silicon wafers. The heterobifunctional crosslinking agentN-succinimidyl-3-(2-pyridyldithio)propionate (SPDP), which contains pyridine rings and disulfide bonds, was involved in the exchange reaction between the sulfhydryl groups and disulfide bonds. With the addition of GSH, the breakage of disulfide bonds was promoted, thereby enhancing the SERS signal of SPDP. GSH can be detected sensitively by detecting the changes in the SPDP signal. The detection limit of GSH is 10 nM, and the method is still highly stable when the external environment is serum or other more complex environments.
Washington Red (WR) dyes and their imaging applications
Chen, Wei,Xian, Ming
, p. 149 - 163 (2020/06/08)
Near-infrared (NIR) fluorescent dyes with optimum photophysical properties are highly useful for bioimaging in living systems, but such dyes are still rare. Recently, our laboratory developed a unique class of NIR fluorescent dyes with high quantum yields and large Stokes shifts. These dyes, named as Washington Red (WR), were found to be useful scaffolds to develop NIR fluorescent imaging probes upon easy modifications on their phenolic hydroxyl group. In this chapter, we provide a comprehensive summary of this work, including the design and synthesis of WR dyes, their photophysical properties studies, and applications in developing NIR probes to image H2S in cells. The detailed experimental protocols are described.
Copper-Catalyzed Direct Synthesis of Aryl Thiols from Aryl Iodides Using Sodium Sulfide Aided by Catalytic 1,2-Ethanedithiol
Xue, Hongyu,Jing, Bing,Liu, Shasha,Chae, Junghyun,Liu, Yajun
, p. 2272 - 2276 (2017/10/06)
A copper-catalyzed direct and effective synthesis of aryl thiols from aryl iodides using readily available Na 2 S·9H 2 O and 1,2-ethanedithiol was described. A variety of aryl thiols were readily obtained in yields of 76-99%. In this protocol, Na 2 S·9H 2 O was used as ultimate sulfur source, and 1,2-ethanedithiol functioned as an indispensable catalytic reagent.
