73708-95-9Relevant articles and documents
Is CuII Coordinated to Patellamides inside Prochloron Cells?
Comba, Peter,Eisenschmidt, Annika,Gahan, Lawrence R.,Herten, Dirk-Peter,Nette, Geoffrey,Schenk, Gerhard,Seefeld, Martin
, p. 12264 - 12274 (2017)
Dinuclear CuII–patellamide complexes (patellamides are naturally occurring cyclic pseudo-octapeptides) are known to be efficient catalysts for hydrolysis reactions of biological importance, for example, those of phosphatase, carbonic anhydrase, and glycosidase. However, the biological role of patellamides is still unknown. Patellamides were originally extracted from the sea squirt Lissoclinum patella, but are now known to be ribosomally expressed by the blue-green algae Prochloron that live in symbiosis with L. patella. In a further step to unravel the metabolic significance of the patellamide complexes, the question as to whether these are also formed inside Prochloron cells is addressed. In this study, a biocompatible patellamide–fluorescent dye conjugate has been introduced into living Prochloron cells and, by means of flow cytometry and confocal microscopy, it is shown that CuII ions are coordinated to patellamides in vivo.
Stable isotope labeled 3-methyl-quinoxaline-2-carboxylic acid and synthesis method thereof
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Paragraph 0032; 0033, (2019/10/01)
The invention discloses a stable isotope labeled 3-methyl-quinoxaline-2-carboxylic acid and a synthesis method thereof. The synthesis method comprises the following steps: S1, acetoacetate reacts withnitrite under the catalysis of organic acid to obtain 2-hydroxyimino-acetoacetate; s2, condensing the 2-hydroxyimino-acetoacetate with stable isotope labeled aniline under the catalysis of acetic acid, and then adding phosphorus oxychloride to react to obtain isotope labeled 3-methyl-quinoxaline-2-carboxylate; s3, hydrolyzing the stable isotope labeled 3-methyl-quinoxaline-2-carboxylic acid esterunder alkaline conditions, adjusting the pH value to acidity after the reaction is finished, and precipitating the stable isotope labeled 3-methyl-quinoxaline-2-carboxylic acid from the aqueous solution. The synthesis method not only has low preparation cost and few experimental steps, but also has purity and isotope abundance of more than 98%, and can be completely used as an internal standard for detecting 3-methyl-quinoxaline-2-carboxylic acid, thus greatly reducing detection cost.
Stereoselective Lewis base-catalyzed asymmetric hydrosilylation of α-acetamido-β-enamino esters: Straightforward approach for the construction of α,β-diamino acid derivatives
Jiang, Yan,Chen, Xing,Hu, Xiao-Yan,Shu, Chang,Zhang, Yong-Hong,Zheng, Yong-Sheng,Lian, Chun-Xia,Yuan, Wei-Cheng,Zhang, Xiao-Mei
supporting information, p. 1931 - 1936 (2013/08/23)
The Lewis base-organocatalyzed asymmetric hydrosilylation of α-acetamido-β-enamino esters was investigated. Among various chiral Lewis base catalysts, a novel catalyst derived from L-serine was found to be the most efficient one which can promote the reaction to afford a series of α,β-diamino acid derivatives with high yields (up to 99%), excellent enantioselectivities (up to 98% ee) and moderate diastereoselectivities (up to 80:20 dr). The absolute configuration of one of the products was determined by the X-ray crystallographic analysis. In addition, the mechanism and the transition state of the reaction were proposed. Copyright
