77874-90-9 Usage
Description
Ellman's anion, also known as 5,5'-dithiobis(2-nitrobenzoic acid) or DTNB, is a chromogenic reagent commonly used in biochemical assays. It is a colorless compound that reacts with thiol groups to form a yellow-colored product, 2-nitro-5-thiobenzoic acid (TNB), which can be easily measured spectrophotometrically. This property makes Ellman's anion a valuable tool for detecting and quantifying thiol-containing compounds in various applications.
Uses
Used in Biochemical Research:
Ellman's anion is used as a chromogenic reagent for the determination of thiols, which are essential components of many biological molecules, including proteins, peptides, and enzymes. The reaction between Ellman's anion and thiol groups allows researchers to accurately measure thiol concentrations in samples, providing valuable insights into the role of thiols in various biological processes.
Used in Covalent Modification of Protein Thiols:
Ellman's anion is used as a reagent for covalent modification of protein thiols, enabling the study of protein function and regulation. By reacting with thiol groups on proteins, Ellman's anion can alter protein activity, stability, and interactions with other molecules. This modification can help researchers understand the role of specific thiol-containing amino acids in protein function and provide insights into the development of new therapeutic agents.
Used in Kinetic Assays of Cholinesterase and Other Enzyme Activities:
Ellman's anion is used as a reagent in kinetic assays to measure the activity of cholinesterase and other enzymes that have thiol groups in their active sites. The reaction between Ellman's anion and the thiol group in the enzyme's active site results in the release of TNB, which can be measured spectrophotometrically. This allows researchers to monitor enzyme activity and kinetics, providing valuable information for understanding enzyme function and developing new drugs targeting these enzymes.
Check Digit Verification of cas no
The CAS Registry Mumber 77874-90-9 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 7,7,8,7 and 4 respectively; the second part has 2 digits, 9 and 0 respectively.
Calculate Digit Verification of CAS Registry Number 77874-90:
(7*7)+(6*7)+(5*8)+(4*7)+(3*4)+(2*9)+(1*0)=189
189 % 10 = 9
So 77874-90-9 is a valid CAS Registry Number.
77874-90-9Relevant articles and documents
Dynamic Behavior of a Two-Site Vesicular Reaction
Moss, Robert A.,Schreck, Ronald P.
, p. 6767 - 6768 (1983)
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Enhanced photoinduced electron transfer at the surface of charged lipid bilayers
Limburg, Bart,Laisne, Guillaume,Bouwman, Elisabeth,Bonnet, Sylvestre
, p. 8965 - 8972 (2014)
Photocatalytic systems often suffer from poor quantum yields due to fast charge recombination: The energy-wasting annihilation of the photochemically created charge-separated state. In this report, we show that the efficiency of photoinduced electron tran
5-(2-Aminoethyl)dithio-2-nitrobenzoate as a more base-stable alternative to ellman's reagent
Zhu, Jinge,Dhimitruka, Ilirian,Pei, Dehua
, p. 3809 - 3812 (2007/10/03)
(Chemical Equation Presented) 5-(2-Aminoethyl)dithio-2-nitrobenzoate (ADNB) reacts with free thiols with kinetics similar to those of Ellman's reagent but has dramatically improved stability under alkaline conditions, making it an excellent alternative to Ellman's reagent for the quantitation of thiol contents and enzymatic assays under basic pH conditions.
