78682-66-3 Usage
Description
H-VAL-AMC, also known as L-Valine 7-amido-4-methylcoumarin, is a synthetic compound with the chemical formula C14H16N2O3. It is characterized by the presence of an L-valine amino acid moiety and a 7-amido-4-methylcoumarin fluorophore. H-VAL-AMC exhibits fluorescent properties upon proteolytic cleavage, making it a valuable tool in various applications.
Uses
Used in Biochemical Research:
H-VAL-AMC is used as a substrate in biochemical research for the detection and quantification of protease activity. H-VAL-AMC is cleaved by proteases, releasing the fluorescent 7-amido-4-methylcoumarin group, which can be measured using fluorescence spectroscopy. This allows for the assessment of enzyme kinetics, substrate specificity, and inhibitor screening.
Used in Drug Discovery and Development:
In the pharmaceutical industry, H-VAL-AMC serves as a valuable tool for drug discovery and development. It is used to identify and optimize potential protease inhibitors, which are important for the treatment of various diseases, including cancer, inflammatory disorders, and infectious diseases. By monitoring the fluorescence emitted upon proteolytic cleavage, researchers can evaluate the potency and selectivity of candidate compounds.
Used in Diagnostic Assays:
H-VAL-AMC is also employed in diagnostic assays for the detection of specific proteases or the assessment of protease activity in biological samples. This can be useful for monitoring disease progression, evaluating the effectiveness of therapeutic interventions, or identifying individuals at risk for certain conditions.
Used in Educational Settings:
In educational settings, H-VAL-AMC can be used as a teaching tool to demonstrate the principles of enzyme kinetics, substrate specificity, and the use of fluorescence as an analytical technique. Students can gain hands-on experience in conducting enzyme assays and interpreting the resulting data.
Check Digit Verification of cas no
The CAS Registry Mumber 78682-66-3 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 7,8,6,8 and 2 respectively; the second part has 2 digits, 6 and 6 respectively.
Calculate Digit Verification of CAS Registry Number 78682-66:
(7*7)+(6*8)+(5*6)+(4*8)+(3*2)+(2*6)+(1*6)=183
183 % 10 = 3
So 78682-66-3 is a valid CAS Registry Number.
78682-66-3Relevant articles and documents
A judgment on postmortem aging in Longissimus dorsi based on a peptide substrate library
Onitsuka, Eiichiro,Okumura, Tomoyuki,Murakami, Hiroshi,Nishino, Norikazu,Morimatsu, Fumiki
, p. 2836 - 2843 (2006)
We attempted to develop a method to determine easily and effectively the degree of postmortem aging of pork longissimus dorsi (LD) by measuring the activity of proteases in the LD using fluorogenic peptide substrates. LD was used to measure the change with time in the protease activity detected with these substrates. Determining the variations within the LD muscles, strong positive correlations were found between changes in hardness and fluorescence intensities against Ac-Ala-MCA, Ac-Met-MCA, Ac-Ser-MCA, Ac-Thr-MCA, and Ac-Ala-Phe-MCA (P 0.005), and strong negative correlations were found between changes in total amounts of free amino acids and Ac-Ala-MCA, Ac-Met-MCA, Ac-Ser-MCA, Ac-Thr-MCA, and Ac-Ala-Phe-MCA (P 0.001). Negative correlations were also observed between changes in the amounts of free Ala, Arg, Lys, Leu, Met, Phe, and Tyr and the fluorescence intensities against Ala, Arg, Lys, Leu, Met, Phe, and Tyr-MCA respectively (P 0.001).
