78783-53-6

Basic information

• Product Name: FPG PROTEIN
• Synonyms: fpg protein from escherichia coli
• CAS NO: 78783-53-6
• Mol File: 78783-53-6.mol
• Article Data: 0

Chemical Properties

• Appearance: /buffered aqueous glycerol solution
• Storage Temp.: −20°C
• CAS DataBase Reference: FPG PROTEIN(CAS DataBase Reference)
• NIST Chemistry Reference: FPG PROTEIN(78783-53-6)
• EPA Substance Registry System: FPG PROTEIN(78783-53-6)

Safety Data

• Hazard Codes: Xi
• Statements: 36/37/38
• Safety Statements: 26-36
• WGK Germany: 1
• Hazardous Substances Data: 78783-53-6(Hazardous Substances Data)

Usage

Description

FPG PROTEIN, also known as Formamidopyrimidine DNA glycosylase, is an enzyme derived from Escherichia coli. It plays a crucial role in the repair of DNA by recognizing and removing oxidized purines, thereby maintaining the integrity and stability of the genetic material.
Used in Research Applications:
FPG PROTEIN is used as a research tool for assessing DNA oxidative damage using the comet assay. This method allows scientists to evaluate the extent of DNA damage caused by reactive oxygen species and other oxidative stressors, providing valuable insights into the mechanisms of DNA repair and the potential effects of various genotoxic agents.

Biochem/physiol Actions

Fpg is a key enzyme in the DNA base excision repair pathway (BER). It catalyses the excision of a broad spectrum of modified purines. Fpg has both DNA glycosylase activity that removes the mutated base and AP-lyase activity that releases ribose leaving both 5′- and 3′-phosphorylated ends in the DNA. The zinc finger motif at its C-terminus is responsible for the DNA binding and AP-lyase activity. In addition, its N-terminal proline acts as a nucleophile to produce a Schiff base intermediate that is essential for enzyme action.