83701-40-0Relevant articles and documents
Azido Functionalized Nucleosides Linked to Controlled Pore Glass as Suitable Starting Materials for Oligonucleotide Synthesis by the Phosphoramidite Approach
Müggenburg, Frederik,Biallas, Alexander,Debiais, Mégane,Smietana, Michael,Müller, Sabine
supporting information, p. 6408 - 6416 (2021/11/30)
It has long been debated whether easily reducible azide groups can withstand the conditions of oligonucleotide synthesis by phosphoramidite chemistry. We have synthesized various 2′- and 3′-azido modified nucleosides and immobilized them on controlled pore glass (CPG) to be used as starting material for the synthesis of oligonucleotides (ONs) with 3′-terminal azide (attached to C2′ or C3′). In a model study, immobilized 3′-azidoadenosine was used as a starting block for the synthesis of a series of oligodeoxynucleotides (ODNs) of increasing length. Upon synthesis, the ODNs were enzymatically digested into monomers and analyzed by RP-HPLC. A peak corresponding to 3′-azidoadenosine was clearly identified in all samples. Quantitative analysis showed that 3′-azidoadenosine was present in nearly the expected ratio to deoxycytidine, which was used as an internal standard. Most importantly, the ratio remained the same for all three ODNs regardless of their length, demonstrating that a higher number of coupling cycles does not lead to higher degradation of the azide. Thus, 2′- or 3′-azido nucleosides attached to a solid support are excellent starting materials for the synthesis of oligonucleotides with 3′-terminal azide.
Efficient access to nonhydrolyzable initiator tRNA based on the synthesis of 3'-azido-3'-deoxyadenosine RNA
Steger, Jessica,Graber, Dagmar,Moroder, Holger,Geiermann, Anna-Skrollan,Aigner, Michaela,Micura, Ronald
supporting information; experimental part, p. 7470 - 7472 (2011/01/04)
Flexibility exercised: Hydrolysis-resistant 3′-aminoacyl-tRNA conjugates that contain a stable amide linkage instead of the natural ester are valuable substrates for biochemical studies of ribosomal processes. In a novel preparation of the stable E. coli initiator tRNA derivative 3′- (Nformylmethionyl) amino-tRNAfMet the key feature is the synthesis of 3′-azido oligoribonucleotides using a new functionalized solid support.
