84325-23-5

Basic information

• Product Name: 4-METHYLUMBELLIFERYL-BETA-D-LACTOSIDE
• Synonyms: 4-MU-BETA-D-LACTOSIDE;4-METHYLUMBELLIFERYL-B-D-LACTOSIDE;4-METHYLUMBELLIFERYL BETA-D-LACTOPYRANOSIDE;4-METHYLUMBELLIFERYL-BETA-D-LACTOSIDE;4-METHYLUMBELLIFERYL-BETA-D-LACTOSIDE, F OR FLUORESCENCE;4-Methylumbelliferyl--D-lactoside;4-methylumbelliferyl β-d-lactopyranoside;4-Methylumbelliferyl β-D-lactopyranoside ,98%
• CAS NO: 84325-23-5
• Molecular Formula: C₂₂H₂₈O₁₃
• Molecular Weight: 500.45
• EINECS: 1533716-785-6
• Mol File: 84325-23-5.mol
• Article Data: 1

Chemical Properties

• Boiling Point: 830.319oC at 760 mmHg
• Flash Point: 286.047oC
• Appearance: /
• Density: 1.658g/cm3
• Vapor Pressure: 0mmHg at 25°C
• Refractive Index: 1.677
• Storage Temp.: 2-8°C
• Solubility: DMF: soluble
• BRN: 8094402
• CAS DataBase Reference: 4-METHYLUMBELLIFERYL-BETA-D-LACTOSIDE(CAS DataBase Reference)
• NIST Chemistry Reference: 4-METHYLUMBELLIFERYL-BETA-D-LACTOSIDE(84325-23-5)
• EPA Substance Registry System: 4-METHYLUMBELLIFERYL-BETA-D-LACTOSIDE(84325-23-5)

Safety Data

• Hazard Codes: Xi
• Statements: 36/37/38
• Safety Statements: 26-36-24/25-22
• WGK Germany: 3
• F: 8-10
• Hazardous Substances Data: 84325-23-5(Hazardous Substances Data)

Usage

Description

4-Methylumbelliferyl-β-D-lactoside, with the chemical abstracts service number 84325-23-5, is a fluorescent coumarin glycoside. It is characterized by its ability to emit fluorescence, which makes it a valuable tool in various analytical and research applications.

Uses

Used in Enzymatic Assays:
4-Methylumbelliferyl-β-D-lactoside is utilized as a chromophoric substrate for enzymes such as cellobiohydrolase I (CBHI) in Trichoderma reesei, cellobiohydrolase I (Cel7A), endoglucanase I (Cel7B), and β-glucosidase. Its use in these enzymatic assays is due to its fluorescent properties, which allow for the easy detection and measurement of enzymatic activity.
Used in the Preparation of Fluorescent Probes:
4-METHYLUMBELLIFERYL-BETA-D-LACTOSIDE is also used in the preparation of fluorescent probes specifically designed for sialidases and sialyltransferases. The reason for its use in this context is its ability to bind to these enzymes and fluoresce upon interaction, providing a sensitive and specific method for detecting and studying the activity of these enzymes.

Biochem/physiol Actions

4-Methylumbelliferyl β-D-lactopyranoside is a fluorescence compound hydrolyzed by the enzyme β-glucosidase (βG) and CBH1

InChI:InChI=1/C22H28O13/c1-8-4-14(25)32-11-5-9(2-3-10(8)11)31-21-19(30)17(28)20(13(7-24)34-21)35-22-18(29)16(27)15(26)12(6-23)33-22/h2-5,12-13,15-24,26-30H,6-7H2,1H3/t12-,13-,15+,16+,17-,18-,19-,20-,21-,22+/m1/s1

Upstream product

• 92052-38-5 2,3,6,2',3',4',6'-hepta-O-acetyl-α-D-lactosyl trichloroacetimidate 
• 90-33-5 7-hydroxy-4-methyl-chromen-2-one 
• 178450-89-0 Acetic acid (2S,3R,4S,5R,6R)-3-acetoxy-6-acetoxymethyl-2-(4-methyl-2-oxo-2H-chromen-7-yloxy)-5-((2S,3R,4S,5S,6R)-3,4,5-triacetoxy-6-acetoxymethyl-tetrahydro-pyran-2-yloxy)-tetrahydro-pyran-4-yl ester 

Downstream Products

• 90-33-5 7-hydroxy-4-methyl-chromen-2-one 

Relevant articles and documents

A multifunctional Pasteurella multocida sialyltransferase: A powerful tool for the synthesis of sialoside libraries

A multifunctional sialyltransferase has been cloned from Pasteurella multocida strain P-1059 and expressed in E. coli as a truncated C-terminal His6-tagged recombinant protein (tPm0188Ph). Biochemical studies indicate that the obtained protein