85933-15-9Relevant articles and documents
IMMOBILIZATION OF ENZYMES INTRODUCING SPACERS. A SYNTHESIS OF CARRIERS WITH SPACERS OF VARIOUS LENGTH
Flemming, Christian,Gabert, Anton,Wand, Helmut,Zemek, Jiri
, p. 184 - 191 (2007/10/02)
A synthesis of spacers of various length and type, utilizing α,ω-dicarboxylic acids, α,ω-diaminoalkanes and succinic anhydride, condensation with carbodiimide, or introduction of -NCS functional groups is described.Carriers with a spacer were prepared by a method of binding the already synthesized spacer to the carrier (glass), and alternatively, by a stepwise synthesis of the spacer on the carrier surface.Carriers containing functional groups (37.2-39.3 μmol/g -NCS and 25-41.5 μmol/g -COOH) prepared in this way had total length of the spacer 0.62-3.92 nm.Whereas the length of the spacer is of no substantial importance for the reaction with low-molecular substances (L-valine, L-cysteine and 2-mercaptoethanol), the optimum length of the spacer for high-molecular compounds (albumin) is about 1.75-2.05 nm.The hydroxyl group adjacent to the functional group of the spacer (1,3-diaminopropan-2-ol) is also of noticeable influence.