86520-53-8Relevant articles and documents
Rapid-throughput competitive colorimetric assay based on monosaccharide-capped gold nanoparticles for detecting lectin-protein interactions
Tsai, Charng-Sheng,Chen, Chao-Tsen
, p. 314 - 322 (2012)
Identification of protein binding partners is one of key challenges in proteomics. A rapid-throughput competitive colorimetric assay is presented that uses gold nanoparticles capped by sugars such as mannopyranoside (Man-GNPs), N-acetylglucosamine (GlcNAc-GNPs), glucose (Glc-GNPs), or N-acetylgalactosamine (GalNAc-GNPs). The assay expediently detects protein-protein interactions in solution, particularly protein-lectin interactions. The competitive assays were conducted in microtiter plates; ten proteins (two glycoproteins and eight lectins) and three sugar-binding lectins (concanavalin A (ConA), wheat germ agglutinin (WGA), and Ricinus communis agglutinin (RCA120)) were combinatorially arranged in a 30-well plate, and constant concentrations of the monosaccharide-capped GNPs (sugar-GNPs) were added to each well. If interactions occurred between the proteins, the sugar-GNPs retained their burgundy color. If no interactions occurred between the proteins, the sugar-GNPs were agglomerated by the corresponding binding lectin, thus producing a blue color. Several new binding pairs were identified for the first time by using this assay, and the binding constants and stoichiometric ratios were determined on the basis of the wavelength shifts. The results were further verified by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and fluorescence resonance energy transfer (FRET) spectroscopy. The assay is very sensitive, requiring only nanomolar protein concentrations.
STREPTOCOCCUS PNEUMONIAE TYPE XIV POLISACCHARIDE: SYNTHESIS OF A REPEATING BRANCHED TETRASACCHARIDE WITH DIOXA-TYPE SPACER-ARMS
Amvam-Zollo, Paul-Henry,Sinay, Pierre
, p. 199 - 212 (2007/10/02)
β-Glycosides of 2-acetamido-2-deoxy-D-glucopyranose were synthesised, using either 7-methoxycarbonyl-3,6-dioxa-1-heptanol or 8-azido-3,6-dioxa-1-octanol.Selective β-lactosylation of 7-methoxycarbonyl-3,6-dioxaheptyl 2-acetamido-3-O-benzyl-2-deoxy-β-D-glucopyranoside with hepta-O-acetyl-lactosyltrichloroacetimidate, followed by β-galactosylation of the secondary hydroxyl group with O-(2,3,4,6-tetra-O-acetyl-α-D-galactopyranosyl)trichloroacetamidate, catalytic hydrogenolysis, and O-deacetylation, gave 7-methoxycarbonyl-3,6-dioxaheptyl 2-acetamido-2-deoxy-4-O-β-D-galactopyranosyl-6-O-(4-O-β-D-galactopyranosyl-β-D-glucopyranosyl)-β-D-glucopyranoside.Selective β-lactosilation of 8-azido-3,6-dioxaoctyl 2-acetamido-3-O-benzyl-2-deoxy-β-D-glucopyranoside with hepta-O-acetyl-lactosyl bromide in the presence of silver triflate, followed by condensation with 2,3,4,6-tetra-O-acetyl-α-D-galactopyranosyl bromide in the presence of silver triflate, catalytic hydrogenolysis, and O-deacetylation, gave 8-azido-3,6-dioxaoctyl 2-acetamido-2-deoxy-4-O-β-D-galactopyranosyl-6-O-(4-O-β-D-galactopyranosyl-β-D-glucopyranosyl)-β-D-glucopyranoside.