9008-96-2

Basic information

• Product Name: PHOSVITIN
• Synonyms: PHOSVITIN;phosvitin from egg yolk
• CAS NO: 9008-96-2
• Molecular Weight: 0
• Product Categories: Phosphorylation Analysis;Post-Translational Modification;Reagents and Controls for Phosphoprotein Analysis
• Mol File: 9008-96-2.mol
• Article Data: 0

Chemical Properties

• Appearance: /
• Storage Temp.: −20°C
• Merck: 13,7451
• CAS DataBase Reference: PHOSVITIN(CAS DataBase Reference)
• NIST Chemistry Reference: PHOSVITIN(9008-96-2)
• EPA Substance Registry System: PHOSVITIN(9008-96-2)

Safety Data

• WGK Germany: 3
• F: 10
• Hazardous Substances Data: 9008-96-2(Hazardous Substances Data)

Usage

Description

Phosvitin is a phosphorylated protein found in egg yolk, known for its high content of serine residues that are phosphorylated. It plays a crucial role in the transport and storage of iron within the egg and exhibits unique properties due to its phosphorylation.

Uses

Used in Biochemistry Research:
Phosvitin is used as a control protein for immobilization on-chip for deep purple protein staining methods, which aids in monitoring post-translation modifications. This application is valuable for studying protein interactions and modifications, contributing to a better understanding of protein functions and regulation.
Used in Enzyme Assays:
Phosvitin serves as a substrate for casein kinase Iε in in vitro casein kinase assays. This use is instrumental in investigating the activity and function of casein kinase Iε, an enzyme involved in various cellular processes.
Used in Analytical Techniques:
Phosvitin is utilized as a standard in gel filtration chromatography (GFC) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) for the quantification of phosvitin from egg extracts. This application ensures accurate measurement and analysis of phosvitin levels, which can be significant in various research and quality control settings.
Used in Spectroscopic Studies:
Phosvitin is employed for immobilization onto Layer-by-Layer (LbL) assemblies for Fourier transform infrared spectroscopy-attenuated total reflectance (FTIR-ATR) studies. This application is beneficial for investigating the structural and functional properties of phosvitin and its interactions with other molecules at the interface.

Biochem/physiol Actions

Phosvitin fed to rats by adding egg yolk to their food resulted in decreased absorption of magnesium, iron and calcium but not of phosphorus.