9012-33-3

Basic information

• Product Name: EC 3.2.1.30
• Synonyms: BETA1-2,3,4,6-N-ACETYLGLUCOSAMINIDASE;BETA1-2,3,4,6-N-ACETYLGLUCOSAMINIDASE, STREPTOCOCCUS PNEUMONIAE;BETA-N-ACETYLGLUCOSAMINIDASE;BETA-N-ACETYLGLUCOSAMINIDASE H;BETA-N-ACETYLHEXOSAMINIDASE;EC 3.2.1.30;EC 3.2.1.52;2-ACETAMIDO-2-DEOXY-BETA-D-GLUCOSIDE ACETAMIDODEOXYGLUCOHYDROLASE
• CAS NO: 9012-33-3
• Molecular Formula: NULL
• Molecular Weight: 0
• EINECS: 232-730-2
• Mol File: 9012-33-3.mol
• Article Data: 0

Chemical Properties

• Appearance: /lyophilized powder
• Density: 1.37[at 20℃]
• Vapor Pressure: 0.004Pa at 25℃
• Storage Temp.: 2-8°C
• Water Solubility: 125g/L at 25℃
• CAS DataBase Reference: EC 3.2.1.30(CAS DataBase Reference)
• NIST Chemistry Reference: EC 3.2.1.30(9012-33-3)
• EPA Substance Registry System: EC 3.2.1.30(9012-33-3)

Safety Data

• Hazard Codes: B
• Statements: 20/22-24-37/38-41-42
• Safety Statements: 53-26-36/37/39-45
• WGK Germany: 3
• F: 10
• Hazardous Substances Data: 9012-33-3(Hazardous Substances Data)

Usage

Uses

Different sources of media describe the Uses of 9012-33-3 differently. You can refer to the following data:
1. β -N-acetylglucosaminidase is a lysosomal enzyme used to hydrolyze N-acetyl-β-D-glucosaminides and N-acetyl-β-Dgalactosaminides. It is used in chemoenzymatic synthesis of oligosaccharides based on their effective transglycosylation of β-GlcNAc and β-GalNAcc. It may be a useful tool to study Alzheimer’s Disease 1 . Acetylglucosaminidase from Canavalia ensiformis has been used to study enzymic detachment of biofilms.
2. Bovine kidney β-N-acetylglucosaminidase is a lysosomal enzyme used to hydrolyze N-acetyl-β-D-glucosaminides and N-acetyl-β-Dgalactosaminides. It is used in chemoenzymatic synthesis of oligosaccharides based on their effective transglycosylation of β-GlcNAc and β-GalNAcc. It may be a useful tool to study Alzheimer′s Disease .

Flammability and Explosibility

Notclassified

Biochem/physiol Actions

β-N-Acetylglucosaminidase from Streptococcus pneumoniae is an extracellular glycosidase and has broad specificity over the β1-3 and β1-6 linkages present in mucins. It belongs to the glycoside hydrolase family 85 (GH85). Also called endo-β-N-acetylglucosaminidase, it cleaves at the asparagine residue of the di-N-acetylchitobiose structure in oligosaccharides and acts on fucosylated N-glycan core. Its deglycosylation functionality is exploited in studying functions of glycoproteins.

Purification Methods

Purify it by chromatography on DEAD-Biogel, hydroxylapatite chromatography and gel filtration through Sephacryl S200. Two isoforms: a hexosaminidase EI with Km 177WM