9012-56-0

Basic information

• Product Name: EC 3.5.1.4
• Synonyms: EC 3.5.1.4;ACYLAMIDE AMIDOHYDROLASE;ACYLASE;ACRYLAMIDE AMIDOHYDROLASE;AMIDASE;AMIDASE BS;AMIDASE, PSEUDOMONAS AERUGINOSA;AMIDASE PSEUDOMONAS AERUGINOSA RECOM-&
• CAS NO: 9012-56-0
• Molecular Weight: 0
• EINECS: 232-736-5
• Mol File: 9012-56-0.mol
• Article Data: 0

Chemical Properties

• Appearance: yellow/solution (slightly hazy)
• Storage Temp.: −20°C
• CAS DataBase Reference: EC 3.5.1.4(CAS DataBase Reference)
• NIST Chemistry Reference: EC 3.5.1.4(9012-56-0)
• EPA Substance Registry System: EC 3.5.1.4(9012-56-0)

Safety Data

• Hazard Codes: Xn
• Statements: 42/43-52/53
• Safety Statements: 22-36/37-45-61
• WGK Germany: 3
• F: 10
• Hazardous Substances Data: 9012-56-0(Hazardous Substances Data)

Usage

Description

EC 3.5.1.4, also known as an amidase, is an enzyme that catalyzes the hydrolysis of amides into an acid and amine. Amidases are a class of hydrolases that play a crucial role in various biological processes and have gained significant attention in biotechnology due to their diverse applications.
Source:
Amidases can be found in various microorganisms, plants, and animals, and can be produced through recombinant DNA technology or isolated from natural sources.
Production Methods:
Amidases can be produced through various methods, including fermentation, extraction from natural sources, or recombinant production in genetically modified organisms. The choice of production method depends on the specific application and desired purity of the enzyme.

Uses

Used in Chemical and Pharmaceutical Industries:
EC 3.5.1.4 is used as a biocatalyst for the production of various chemicals and pharmaceuticals. Amidases can efficiently catalyze the synthesis of amides, esters, and peptides, making them valuable tools in the synthesis of drugs, agrochemicals, and other specialty chemicals.
Used in Bioremediation:
Amidases are used in the bioremediation of industrial effluents and contaminated sites. They can break down toxic compounds, such as acrylamide, into less harmful substances, thus reducing the environmental impact of industrial processes.
Used in Wastewater Treatment:
EC 3.5.1.4 is used as a treatment for the elimination of metal ions in wastewater. Amidases can bind to metal ions, forming stable complexes that can be easily removed from wastewater, thus reducing the concentration of toxic metal ions in the environment.
Used in Immobilized Enzyme Technology:
Immobilized amidase can be used efficiently for the production of acrylic acid from acrylamide, converting a toxic ambient contaminant into a widely used industrial raw material. Immobilization of amidases on solid supports allows for their reuse and increased stability, making the process more cost-effective and environmentally friendly.

Biochem/physiol Actions

The amidase from Pseudomonas aeruginosa isa 6 × 38-kDa enzyme that catalyzes the hydrolysis of a small range of short aliphatic amides. Each amidase monomer is formed by a globular four-layer αββα sandwich domain with an additional 81-residue long C-terminal segment .