9013-53-0

Basic information

• Product Name: EC 3.1.31.1
• Synonyms: EC 3.1.31.1;nuclease micrococcal from staphylococcus aureus;Nuclease, Staphylococcus aureus;NUCLEASE MICROCOCCAL FROM STAPHYLOCOCCU;endonuclease micrococcal;nuclease micrococcal, Staphylococcus aureus;Endonuclease micrococcal, Micrococcal endonuclease, MNase;Micrococcal Nuteus
• CAS NO: 9013-53-0
• Molecular Weight: 0
• EINECS: 232-748-0
• Mol File: 9013-53-0.mol
• Article Data: 0

Chemical Properties

• Appearance: /
• Storage Temp.: −20°C
• CAS DataBase Reference: EC 3.1.31.1(CAS DataBase Reference)
• NIST Chemistry Reference: EC 3.1.31.1(9013-53-0)
• EPA Substance Registry System: EC 3.1.31.1(9013-53-0)

Safety Data

• Safety Statements: 22-24/25
• WGK Germany: 3
• F: 10-21
• Hazardous Substances Data: 9013-53-0(Hazardous Substances Data)

Usage

Description

EC 3.1.31.1 is a nuclease enzyme that is involved in the degradation of nucleic acids. It has been identified in various organisms, including Staphylococcus aureus and Micrococcus luteus, and has been studied for its potential applications in molecular biology and biotechnology.

Uses

Used in Molecular Biology Research:
EC 3.1.31.1 is used as a tool for studying the expression characteristics of genes and the location of nucleosomes in chromatin. It has been used in studies to assess coagulase and heat-resistant strains of S. aureus found in animals, as well as to investigate the expression of two genes in S. aureus that encode two thermostable nucleases.
Used in Ribonucleic Acid (RNA) Research:
EC 3.1.31.1, specifically Nuclease S7, is used in the ribonucleic acid (RNA) selection process for ribosome profiling. This process allows for the identification of specific RNA molecules and their interactions with ribosomes, providing valuable insights into gene expression and regulation.
Used in Biotechnology Applications:
EC 3.1.31.1 has potential applications in biotechnology, particularly in the development of novel tools and techniques for nucleic acid manipulation and analysis. Its ability to degrade nucleic acids selectively makes it a valuable enzyme for various molecular biology applications, including DNA fingerprinting, gene expression analysis, and the development of diagnostic tools.

Biochem/physiol Actions

Cleavage of DNA by Nuclease micrococcal is greater at the 5′ sides of A or T nucleotides than at G or C.