9014-00-0

Basic information

• Product Name: EC 1.14.14.3
• Synonyms: LUCIFERASE, BACTERIAL;LUCIFERASE;BACTERIAL LUCIFERASE;EC 1.14.14.3;LUCIFERASE FROM PHOTOBACT. FISCHERI, LYO PHIL., POWDER;LUCIFERASE BACTERIAL FROM VIBRIO*FISCHER I;LUCIFERASE FIREFLY RECOMBINANT 95+%;LUCIFERASE FROM PH. FISCHERI PARTIALLY PURIFIED POWDER
• CAS NO: 9014-00-0
• Molecular Weight: 0
• EINECS: 232-751-7
• Mol File: 9014-00-0.mol
• Article Data: 0

Chemical Properties

• Appearance: /powder
• Storage Temp.: −20°C
• CAS DataBase Reference: EC 1.14.14.3(CAS DataBase Reference)
• NIST Chemistry Reference: EC 1.14.14.3(9014-00-0)
• EPA Substance Registry System: EC 1.14.14.3(9014-00-0)

Safety Data

• WGK Germany: 3
• F: 3-10-21
• Hazardous Substances Data: 9014-00-0(Hazardous Substances Data)

Usage

Description

EC 1.14.14.3, also known as Alkanal monooxygenase, is an enzyme that catalyzes the oxidation of aliphatic aldehydes to their corresponding carboxylic acids. It is involved in the metabolism of various aldehydes and plays a crucial role in detoxification processes.

Uses

Used in Biomedical Research:
EC 1.14.14.3 is used as a research tool for studying the bioluminescence imaging of mammary tumor cryosections, providing valuable insights into tumor growth and progression.
Used in Biochemical Assays:
In the field of biochemistry, EC 1.14.14.3 is used as a component of the luciferase reaction mixture, enabling the assessment of light emission and oxygen consumption by bioluminescent bacteria, such as Vibrio fischeri.
Used in Microbiology:
In microbiology, EC 1.14.14.3 is utilized in Mix B respiration medium to determine the ATP synthetic activity of the H+-ATP synthase, contributing to the understanding of cellular energy production.
Used in Enzyme Assays:
The enzyme is also used in thermal aggregation assays to evaluate the activity of CnoX (YbbN), an important enzyme involved in bacterial metabolism.

Biochem/physiol Actions

Luciferase catalyzes the conversion of reduced flavin mononucleotide in the presence of oxygen and long-chain aldehyde to flavin mononucleotide, visible light and a fatty acid. It is a reporter enzyme for sensitive quantitation. The lucifearse fusion proteins have been useful in many protein based interaction studies especially in living cells.