9015-73-0

Basic information

• Product Name: DEAE-DEXTRAN
• Synonyms: deae-d;diethylaminoethyl-dextrapolymer;N,N-DIETHYLAMINOETHYL DEXTRAN;DEXTRAN, DEAE ETHER;DEXTRAN, DIETHYLAMINOETHYL ETHER;DIETHYLAMINOETHYL DEXTRAN;DIETHYLAMINOETHYL-DEXTRAN CHLORIDE FORM;DEAE-DEXTRAN
• CAS NO: 9015-73-0
• Molecular Weight: 0
• Mol File: 9015-73-0.mol
• Article Data: 0

Chemical Properties

• Boiling Point: 273.6°C at 760 mmHg
• Flash Point: 69.9°C
• Appearance: white/powder
• Density: 0.874g/cm³
• Vapor Pressure: 0.00568mmHg at 25°C
• Refractive Index: 1.452
• Storage Temp.: ?20°C
• Solubility: H2O: 0.1 g/mL, clear
• Water Solubility: Soluble in water.
• CAS DataBase Reference: DEAE-DEXTRAN(CAS DataBase Reference)
• NIST Chemistry Reference: DEAE-DEXTRAN(9015-73-0)
• EPA Substance Registry System: DEAE-DEXTRAN(9015-73-0)

Safety Data

• WGK Germany: 3
• RTECS: HH9250000
• F: 3
• Hazardous Substances Data: 9015-73-0(Hazardous Substances Data)

Usage

Description

DEAE-Dextran, also known as Diethylaminoethyl-dextran, is a polycationic derivative of the carbohydrate polymer dextran. It is a cationic bile acid sequestrant that can tightly associate with the negatively charged backbone of nucleic acids, allowing it to adhere to the cell membrane and enter into the cytoplasm via endocytosis or osmotic shock.

Uses

Used in Pharmaceutical Industry:
DEAE-Dextran is used as an efficient adjuvant in vaccine production for immunization of animals with inactivated viruses, such as foot-and-mouth disease and Venezuelan equine encephalomyelitis virus. It may stimulate helper T-cell function in antibody synthesis, resulting in a typical IgM-IgG response.
Used in Biotechnology Industry:
DEAE-Dextran 500 is used as a polycationic stabilizing molecule and transfection reagent in the development of sustained release protein delivery and nucleic acid transfection systems. It may also be used for cell immobilization in biosensors.
Used in Research:
DEAE-Dextran is used as a support for ion-exchange chromatography and as a component/adjuvant in transfection and gene therapy, cholesterol/weight modulation, protein stabilization, drug delivery, and as a flocculant.
Used in Cell Culture:
DEAE-Dextran is used for transfection in mammalian cell culture, suitable for transient expression of cloned genes but not for stable transformation of cells. It is particularly useful for certain cell types, such as CV-1 and COS cells, that cannot be exposed to calcium phosphate due to its toxicity. However, it is important to note that DEAE-Dextran is also toxic for cells, limiting its use to specific cell types and requiring reduced serum media during the transfection procedure.

Chemical and Biological properties

DEAE-Dextran offers a wide range of chemical and biological properties. The following properties are well documented: ① Adjuvant in vaccines ② Agent for transfection ③ Stabilizer of proteins ④ Agent for reduction of cholesterol count ⑤ Enhancer of viral infectivity

Dextran derivatives

DEAE-Dextran, being a polycation, offers many applications, such as a component/adjuvant in vaccines, for transfection and gene therapy, cholesterol/weight modulation, a stabiliser of proteins, in drug delivery and as a flocculant. DEAE-Dextran is made by reaction of dextran with (2-chloroethyl) diethylammoniumchlorid in alkaline solution. DEAE - Dextran contains three basic groups with different pKa values. There are two tertiary groups, one in the single substituted glucose unit having a pKa value of approximately 9.2 and the tertiary group in the tandem substitution having a pKa value of approximately 5.5. The quaternary amino group in the tandem substitution has a pKa value ~ 14. Like the polyanions, the polycations also exhibit a wide range of interesting biological properties. In particular, DEAE-dextrans appear to enhance a number of cellular processes. Many reports (398-401) testify to the enhancement of cellular uptake of viral RNA and also intact virus particles. Considerable interest has been focussed on the enhanced production of interferon by polyribonucleotide complexes in the presence of DEAE-dextran (402–404). These processes appear to operate without detrimental effects on the viability of the cells (398). To what extent these effects are due to the interaction between the cell surface (net negative charge) and the polycation is uncertain. For most of these studies, a DEAE-dextran with MW approx. 500 000 and DS 0.5 has been used at concentrations from 10–500 μg/ml. DEAE-Dextran 500 is a polycationic derivative of dextran, produced from dextran with an average molecular weight >500,000 Da. DEAE-Dextran 500 has a broad variety of applications including medicinal products, technical and chromatographic applications and as a polycationic stabilizing molecule. DEAE-Dextran 500 is supplied in its HCI form.

InChI:InChI=1/C12H28N2O/c1-5-13(6-2)9-11-15-12-10-14(7-3)8-4/h5-12H2,1-4H3