9015-85-4

Basic information

• Product Name: T4 DNA LIGASE
• Synonyms: T4 DNA LIGASE;RNA LIGASE, T4 PAHGE;DNA LIGASE;DNA LIGASE NAD-DEPENDENT;DNA LIGASE T4;DNA LIGASE, THERMOSTABLE;EC 6.5.1.1;EC 6.5.1.2
• CAS NO: 9015-85-4
• Molecular Weight: 0
• EINECS: 232-770-0
• Product Categories: Enzyme
• Mol File: 9015-85-4.mol
• Article Data: 0

Chemical Properties

• Appearance: /buffered aqueous glycerol solution
• Storage Temp.: −20°C
• CAS DataBase Reference: T4 DNA LIGASE(CAS DataBase Reference)
• NIST Chemistry Reference: T4 DNA LIGASE(9015-85-4)
• EPA Substance Registry System: T4 DNA LIGASE(9015-85-4)

Safety Data

• WGK Germany: 3
• F: 10
• TSCA: Yes
• Hazardous Substances Data: 9015-85-4(Hazardous Substances Data)

Usage

Description

T4 DNA Ligase, also known as Escherichia coli T4 DNA Ligase, is an enzyme derived from the bacteriophage T4 that infects E. coli bacteria. It is a crucial component in molecular biology and genetic engineering, playing a significant role in the manipulation and analysis of DNA molecules. T4 DNA Ligase possesses the ability to catalyze the formation of phosphodiester bonds between neighboring 3′-hydroxyland 5′-phosphate ends in double-stranded DNA, as well as closing single-stranded nicks in double-stranded DNA. This enzyme is supplied with a 10x concentrated ligation buffer that includes ATP, which is essential for its function.

Uses

Used in Molecular Biology and Genetic Engineering:
T4 DNA Ligase is used as a molecular biology tool for forming energy-dependent phosphodiester linkages between the termini of adjacent polynucleotides of duplex DNA. This process is vital for various applications, such as the ligation of cloning vector and restriction insert fragments, which are essential steps in cloning, gene expression, and DNA repair mechanisms.
Used in DNA Cloning:
In the field of DNA cloning, T4 DNA Ligase is employed as a critical enzyme for joining DNA fragments together. It is used to ligate the ends of DNA molecules, such as those generated by restriction enzymes, allowing for the creation of recombinant DNA molecules that can be introduced into host organisms for expression or further analysis.
Used in DNA Repair:
T4 DNA Ligase plays a significant role in DNA repair mechanisms, as it is capable of sealing single-stranded nicks in double-stranded DNA. This function is essential for maintaining the integrity and stability of the genetic material, preventing mutations and ensuring proper cellular function.
Used in Next-Generation Sequencing (NGS):
T4 DNA Ligase is also utilized in next-generation sequencing (NGS) technologies, where it is employed to ligate specific adapters or barcodes to DNA fragments. This process enables the simultaneous sequencing of multiple samples in a single run, allowing for high-throughput and cost-effective genetic analysis.
Used in Synthetic Biology:
In the emerging field of synthetic biology, T4 DNA Ligase is used as a key enzyme for assembling genetic circuits and designing novel biological systems. It facilitates the construction of complex DNA structures and the integration of multiple genetic elements, paving the way for innovative applications in biotechnology and medicine.